Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸ 10 µg 110 563 ₸

Order now and get it on Wednesday March 10, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Mouse monoclonal [GI191E] to Bcl6
Suitable for: WB, IP
Reacts with: Mouse, Human

Product name

Anti-Bcl6 antibody [GI191E]
See all Bcl6 primary antibodies
Description

Mouse monoclonal [GI191E] to Bcl6
Host species

Mouse
Tested Applications & Species

Application Species
IP
Human

WB
Mouse

Human

See all applications and species data
Immunogen

Recombinant fragment within Human Bcl6 aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P41182

Run BLAST with Run BLAST with
Positive control
- WB: Raji and Ramos whole cell lysate. Mouse brain, heart and kidney tissue lysate. IP: Ramos whole cell lysate.
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Application	Species
IP	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

GI191E
Isotype

IgG1
Research areas

Western blot - Anti-Bcl6 antibody [GI191E] (ab241549)

All lanes : Anti-Bcl6 antibody [GI191E] (ab241549) at 1/1000 dilution

Lane 1 : Ramos (Human Burkitt’s lymphoma B lymphocyte), whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 79 kDa

Exposure time: 15 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Bcl6 antibody [GI191E] (ab241549)

All lanes : Anti-Bcl6 antibody [GI191E] (ab241549) at 1/1000 dilution

Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse kidney tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

Predicted band size: 79 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lane 1,3: 81 secs; Lane 2: 5 secs.
Immunoprecipitation - Anti-Bcl6 antibody [GI191E] (ab241549)

Bcl6 was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell lysate with ab241549 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab241549 at 1/500 dilution. Anti-mouse IgG for IP (HRP) (ab131368) was used at 1/1000 dilution.

Lane 1: Ramos whole cell lysate 10µg (Input).
Lane 2: ab241549 IP in Ramos whole cell lysate.
Lane 3: Mouse monoclonal IgG instead of ab241549 in Ramos whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
Anti-Bcl6 antibody [GI191E] (ab241549)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com