Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Bcl6 antibody [GI191E] - BSA and Azide free (ab252801)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [GI191E] to Bcl6 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Bcl6 antibody [GI191E] - BSA and Azide free
    See all Bcl6 primary antibodies

  • Description

    Mouse monoclonal [GI191E] to Bcl6 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment within Human Bcl6 aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P41182

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Raji and Ramos whole cell lysate. Mouse brain, heart and kidney tissue lysate. IP: Ramos whole cell lysate.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab252801 is the carrier-free version of ab241549. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-Bcl6 antibody [GI191E] - BSA and Azide free (ab252801)
    Western blot - Anti-Bcl6 antibody [GI191E] - BSA and Azide free (ab252801)
    All lanes : Anti-Bcl6 antibody [GI191E] (ab241549) at 1/1000 dilution

    Lane 1 : Ramos (Human Burkitt’s lymphoma B lymphocyte), whole cell lysate
    Lane 2 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 79 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab241549).

  • Western blot - Anti-Bcl6 antibody [GI191E] - BSA and Azide free (ab252801)
    Western blot - Anti-Bcl6 antibody [GI191E] - BSA and Azide free (ab252801)
    All lanes : Anti-Bcl6 antibody [GI191E] (ab241549) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 79 kDa



    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab241549).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1,3: 81 secs; Lane 2: 5 secs.

  • Immunoprecipitation - Anti-Bcl6 antibody [GI191E] - BSA and Azide free (ab252801)
    Immunoprecipitation - Anti-Bcl6 antibody [GI191E] - BSA and Azide free (ab252801)

    This image was produced using the same antibody clone but in a different formulation ab241549, PBS, sodium azide, glycerol and BSA.

    Bcl6 was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell lysate with ab241549 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab241549 at 1/500 dilution. Anti-mouse IgG for IP (HRP) (ab131368) was used at 1/1000 dilution.

    Lane 1: Ramos whole cell lysate 10µg (Input).
    Lane 2: ab241549 IP in Ramos whole cell lysate.
    Lane 3: Mouse monoclonal IgG instead of ab241549 in Ramos whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 seconds.

  • Anti-Bcl6 antibody [GI191E] - BSA and Azide free (ab252801)
    Anti-Bcl6 antibody [GI191E] - BSA and Azide free (ab252801)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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