Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Wednesday March 17, 2021

Anti-Mesothelin antibody [EPR17823-69] (ab213174)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17823-69] to Mesothelin
Suitable for: WB, IP
Reacts with: Mouse

Product name

Anti-Mesothelin antibody [EPR17823-69]
See all Mesothelin primary antibodies
Description

Rabbit monoclonal [EPR17823-69] to Mesothelin
Host species

Rabbit
Specificity

This antibody recognizes a different epitope of Mesothelin compared to ab187063.
Tested Applications & Species

Application Species
IP
Mouse

WB
Mouse

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: His-tagged mouse mesothelin fragment recombinant protein (aa298-600); NIH/3T3 whole cell lysate; mouse lung lysate. IP: NIH/3T3 whole cell lysate.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
IP	Mouse
WB	Mouse

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17823-69
Isotype

IgG
Research areas

Western blot - Anti-Mesothelin antibody [EPR17823-69] (ab213174)

Lane 1 : Anti-Mesothelin antibody [EPR17823-69] (ab213174) at 1/20000 dilution
Lane 2 : Anti-Mesothelin antibody [EPR17823-69] (ab213174) at 1/100000 dilution

All lanes : His-tagged mouse mesothelin fragment recombinant protein (aa298-600) 10 ng

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Developed using the ECL technique.

Predicted band size: 69 kDa
Observed band size: 40 kDa
why is the actual band size different from the predicted?

Exposure time: 1 minute

Blocking and dilution buffer: 5% NFDM /TBST.
Immunoprecipitation - Anti-Mesothelin antibody [EPR17823-69] (ab213174)

Mesothelin was immunoprecipitated from 1 mg NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate with ab213174 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab213174 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

Lane 1: NIH/3T3 whole cell lysate 10 μg (Input).
Lane 2: ab213174 IP NIH/3T3 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213174 in NIH/3T3 whole cell lysate (-)

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 seconds.
Western blot - Anti-Mesothelin antibody [EPR17823-69] (ab213174)

All lanes : Anti-Mesothelin antibody [EPR17823-69] (ab213174) at 1/1000 dilution

Lane 1 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 2 : Mouse lung lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Developed using the ECL technique.

Predicted band size: 69 kDa
Observed band size: 40,71 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking and dilution buffer: 5% NFDM /TBST.

The expression profile observed is consistent with what has been described in the literature (PMID: 16857795; PMID: 17332303).
Anti-Mesothelin antibody [EPR17823-69] (ab213174)

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