Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Mesothelin antibody [EPR17823-52] (ab187063)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17823-52] to Mesothelin
  • Suitable for: WB, IP, Flow Cyt
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Mesothelin antibody [EPR17823-52]
    See all Mesothelin primary antibodies

  • Description

    Rabbit monoclonal [EPR17823-52] to Mesothelin

  • Host species

    Rabbit

  • Specificity

    This antibody recognizes a different epitope of Mesothelin compared to ab213174.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His-tagged mouse mesothelin fragment recombinant protein (aa298-600); NIH/3T3 whole cell lysate; Mouse lung and heart lysates; IP: NIH/3T3 whole cell lysate; Flow Cyt: NIH/3T3 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Mesothelin antibody [EPR17823-52] (ab187063)
    Western blot - Anti-Mesothelin antibody [EPR17823-52] (ab187063)
    Lane 1 : Anti-Mesothelin antibody [EPR17823-52] (ab187063) at 1/1000 dilution
    Lane 2 : Anti-Mesothelin antibody [EPR17823-52] (ab187063) at 1/5000 dilution
    Lane 3 : Anti-Mesothelin antibody [EPR17823-52] (ab187063) at 1/20000 dilution

    All lanes : His-tagged mouse mesothelin fragment recombinant protein (aa298-600)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 69 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-Mesothelin antibody [EPR17823-52] (ab187063)
    Flow Cytometry - Anti-Mesothelin antibody [EPR17823-52] (ab187063)

    Flow Cytometry analysis of NIH/3T3 (mouse embryo fibroblast cell line) cells labeling Mesothelin with ab187063 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 was used as the secondary antibody (red). Rabbit monoclonal IgG (ab172730) was used as the isotype control (black). Blue - Unlabeled control, cells without incubation with primary and secondary antibodies.

  • Immunoprecipitation - Anti-Mesothelin antibody [EPR17823-52] (ab187063)
    Immunoprecipitation - Anti-Mesothelin antibody [EPR17823-52] (ab187063)

    Mesothelin was immunoprecipitated from 1 mg of NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate with ab187063 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab187063 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: NIH/3T3 whole cell lysate 10 µg (Input). 

    Lane 2: ab187063 IP in NIH/3T3 whole cell lysate (+). 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab187063 in NIH/3T3 whole cell lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Western blot - Anti-Mesothelin antibody [EPR17823-52] (ab187063)
    Western blot - Anti-Mesothelin antibody [EPR17823-52] (ab187063)
    All lanes : Anti-Mesothelin antibody [EPR17823-52] (ab187063) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 2 : Mouse lung lysate
    Lane 3 : Mouse heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 69 kDa
    Observed band size: 40,71 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 16857795; PMID: 17332303).

  • Anti-Mesothelin antibody [EPR17823-52] (ab187063)
    Anti-Mesothelin antibody [EPR17823-52] (ab187063)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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