Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Mesothelin antibody [EPR17823-52] (ab187063)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17823-52] to Mesothelin
Suitable for: WB, IP, Flow Cyt
Reacts with: Mouse

Product name

Anti-Mesothelin antibody [EPR17823-52]
See all Mesothelin primary antibodies
Description

Rabbit monoclonal [EPR17823-52] to Mesothelin
Host species

Rabbit
Specificity

This antibody recognizes a different epitope of Mesothelin compared to ab213174.

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
IP	Mouse
WB	Mouse

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: His-tagged mouse mesothelin fragment recombinant protein (aa298-600); NIH/3T3 whole cell lysate; Mouse lung and heart lysates; IP: NIH/3T3 whole cell lysate; Flow Cyt: NIH/3T3 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17823-52
Isotype

IgG
Research areas

Western blot - Anti-Mesothelin antibody [EPR17823-52] (ab187063)

Lane 1 : Anti-Mesothelin antibody [EPR17823-52] (ab187063) at 1/1000 dilution
Lane 2 : Anti-Mesothelin antibody [EPR17823-52] (ab187063) at 1/5000 dilution
Lane 3 : Anti-Mesothelin antibody [EPR17823-52] (ab187063) at 1/20000 dilution

All lanes : His-tagged mouse mesothelin fragment recombinant protein (aa298-600)

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Developed using the ECL technique.

Predicted band size: 69 kDa
Observed band size: 40 kDa
why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.
Flow Cytometry - Anti-Mesothelin antibody [EPR17823-52] (ab187063)

Flow Cytometry analysis of NIH/3T3 (mouse embryo fibroblast cell line) cells labeling Mesothelin with ab187063 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 was used as the secondary antibody (red). Rabbit monoclonal IgG (ab172730) was used as the isotype control (black). Blue - Unlabeled control, cells without incubation with primary and secondary antibodies.
Immunoprecipitation - Anti-Mesothelin antibody [EPR17823-52] (ab187063)

Mesothelin was immunoprecipitated from 1 mg of NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate with ab187063 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab187063 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: NIH/3T3 whole cell lysate 10 µg (Input).

Lane 2: ab187063 IP in NIH/3T3 whole cell lysate (+).

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab187063 in NIH/3T3 whole cell lysate (-).

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.
Western blot - Anti-Mesothelin antibody [EPR17823-52] (ab187063)

All lanes : Anti-Mesothelin antibody [EPR17823-52] (ab187063) at 1/1000 dilution

Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : Mouse lung lysate
Lane 3 : Mouse heart lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Developed using the ECL technique.

Predicted band size: 69 kDa
Observed band size: 40,71 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID: 16857795; PMID: 17332303).
Anti-Mesothelin antibody [EPR17823-52] (ab187063)

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