Antibodies, Proteins, Kits and Reagents for Life Science

281 433 ₸ Product size

100 µl 281 433 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Mesothelin antibody [EPR19025-42] (ab196235)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19025-42] to Mesothelin
Suitable for: IP, Flow Cyt, IHC-P, WB
Reacts with: Human

Product name

Anti-Mesothelin antibody [EPR19025-42]
See all Mesothelin primary antibodies
Description

Rabbit monoclonal [EPR19025-42] to Mesothelin
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa cell lysate; Human ovary cancer lysate. IHC-P: Human mesothelioma and ovarian adenocarcinoma tissues. Flow Cyt: HeLa cells. IP: HeLa whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19025-42
Isotype

IgG
Research areas

Flow Cytometry - Anti-Mesothelin antibody [EPR19025-42] (ab196235)

Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Mesothelin with ab196235 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Total viable cells were gated for the image.
Western blot - Anti-Mesothelin antibody [EPR19025-42] (ab196235)

All lanes : Anti-Mesothelin antibody [EPR19025-42] (ab196235) at 1/1000 dilution

Lane 1 : Human ovary cancer lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 69 kDa
Observed band size: 40,69 kDa
why is the actual band size different from the predicted?

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular mass is consistent with what has been described in the literature (PMID: 24146039).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR19025-42] (ab196235)

Immunohistochemical analysis of paraffin-embedded human mesothelioma tissue labeling Mesothelin with ab196235 at 1/1500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining on human mesothelioma (PMID: 17945478). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR19025-42] (ab196235)

Immunohistochemical analysis of paraffin-embedded human ovarian adenocarcinoma tissue labeling Mesothelin with ab196235 at 1/1500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on tumor cells of human ovarian adenocarcinoma (PMID: 17945478). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-Mesothelin antibody [EPR19025-42] (ab196235)

Mesothelin was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) lysate with ab196235 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab196235 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: HeLa whole cell lysate 10 µg (Input).

Lane 2: ab196235 IP in HeLa whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab196235 in HeLa whole cell lysate.

Exposure time: 3 minutes.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Anti-Mesothelin antibody [EPR19025-42] (ab196235)

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