Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Mesothelin antibody [EPR2685(2)] (ab134109)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2685(2)] to Mesothelin
Suitable for: WB, IP, IHC-P
Reacts with: Human

Product name

Anti-Mesothelin antibody [EPR2685(2)]
See all Mesothelin primary antibodies
Description

Rabbit monoclonal [EPR2685(2)] to Mesothelin
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Mesothelin aa 550 to the C-terminus. The exact sequence is proprietary.
Database link: Q13421
Positive control
- WB: HeLa whole cell lysate (ab150035); Human fetal lung lysate. IHC-P: Human mesothelioma and ovarian carcinoma tissue.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 5.50 x 10 ^-12 M
Learn more about K_D
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR2685(2)
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR2685(2)] (ab134109)

Immunohistochemical staining of paraffin embedded human skeletal muscle with purified ab134109 at a working dilution of 1/800. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Western blot - Anti-Mesothelin antibody [EPR2685(2)] (ab134109)

Anti-Mesothelin antibody [EPR2685(2)] (ab134109) at 1/1000 dilution (purified) + HeLa whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 69 kDa
Observed band size: 69 kDa
Additional bands at: 40 kDa (possible cleavage fragment)

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunoprecipitation - Anti-Mesothelin antibody [EPR2685(2)] (ab134109)

ab134109 (purified) at 1/40 immunoprecipitating mesothelin in 10 μg HeLa cell lysate (Lanes 1 and 2, observed at 40 and 69 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR2685(2)] (ab134109)

Immunohistochemical staining of paraffin embedded human mesothelioma with purified ab134109 at a working dilution of 1/800. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Western blot - Anti-Mesothelin antibody [EPR2685(2)] (ab134109)

All lanes : Anti-Mesothelin antibody [EPR2685(2)] (ab134109) at 1/1000 dilution (unpurified)

Lane 1 : HeLa cell lysate
Lane 2 : Human fetal lung lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 69 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR2685(2)] (ab134109)

Immunohistochemical analysis of paraffin embedded Human mesothelioma tissue labelling Mesothelin with unpurified ab134109 at 1/1000.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR2685(2)] (ab134109)

Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue labelling Mesothelin with unpurified ab134109 at 1/1000.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR2685(2)] (ab134109)

Immunohistochemical analysis of paraffin embedded normal Human pancreas tissue using unpurified ab134109 showing -ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR2685(2)] (ab134109)

Immunohistochemical analysis of paraffin embedded Human Pancreatic carcinoma tissue using unpurified ab134109 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR2685(2)] (ab134109)

Immunohistochemical analysis of paraffin embedded Human Greator omentum tissue using unpurified ab134109 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
OI-RD Scanning - Anti-Mesothelin antibody [EPR2685(2)] (ab134109)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-Mesothelin antibody [EPR2685(2)] (ab134109)

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