Anti-PELP1 antibody [EPR15212] (ab187653)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15212] to PELP1
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
Overview
-
Product name
Anti-PELP1 antibody [EPR15212]
See all PELP1 primary antibodies -
Description
Rabbit monoclonal [EPR15212] to PELP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P RatHumanWB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- MDA-MB-435, MCF-7 and 293T cell lysates. Human breast carcinoma and Rat brain tissue.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15212 -
Isotype
IgG -
Research areas
Images
-
Immunohistochemical analysis of formalin fixed and paraffin embedded rat brain tissue labeling PELP1 with ab187653 at 1/100 dilution, counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of formalin fixed and paraffin embedded Human breast carcinoma tissue labeling PELP1 with ab187653 at 1/100 dilution, counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
All lanes : Anti-PELP1 antibody [EPR15212] (ab187653) at 1/5000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 120 kDa
-
Anti-PELP1 antibody [EPR15212] (ab187653) at 1/2000 dilution + MDA-MB-435 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 120 kDa
-