Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Wednesday March 03, 2021

Anti-PELP1 antibody [EPR15212] (ab187653)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15212] to PELP1
Suitable for: IHC-P, WB
Reacts with: Rat, Human

Product name

Anti-PELP1 antibody [EPR15212]
See all PELP1 primary antibodies
Description

Rabbit monoclonal [EPR15212] to PELP1
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Rat

Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- MDA-MB-435, MCF-7 and 293T cell lysates. Human breast carcinoma and Rat brain tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Rat Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15212
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PELP1 antibody [EPR15212] (ab187653)

Immunohistochemical analysis of formalin fixed and paraffin embedded rat brain tissue labeling PELP1 with ab187653 at 1/100 dilution, counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PELP1 antibody [EPR15212] (ab187653)

Immunohistochemical analysis of formalin fixed and paraffin embedded Human breast carcinoma tissue labeling PELP1 with ab187653 at 1/100 dilution, counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-PELP1 antibody [EPR15212] (ab187653)

All lanes : Anti-PELP1 antibody [EPR15212] (ab187653) at 1/5000 dilution

Lane 1 : MCF-7 cell lysate
Lane 2 : 293T cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 120 kDa
Western blot - Anti-PELP1 antibody [EPR15212] (ab187653)

Anti-PELP1 antibody [EPR15212] (ab187653) at 1/2000 dilution + MDA-MB-435 cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 120 kDa
Anti-PELP1 antibody [EPR15212] (ab187653)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com