Anti-NRBF2 antibody (ab85733)
Key features and details
- Rabbit polyclonal to NRBF2
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NRBF2 antibody
See all NRBF2 primary antibodies -
Description
Rabbit polyclonal to NRBF2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region between residue 237 and 287 of Human NRBF2 (NP_110386.2).
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Positive control
- Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NRBF2 antibody (ab85733)All lanes : Anti-NRBF2 antibody (ab85733) at 0.4 µg/ml
Lane 1 : Whole cell
lysate from HeLa cells at 50 µg
Lane 2 : Whole cell
lysate from HeLa cells at 15 µg
Lane 3 : Whole cell
lysate from HeLa cells at 5 µg
Lane 4 : Whole cell
lysate from 293T cells at 50 µg
Lane 5 : Whole cell
lysate from NIH3T3 cells at 50 µg
Developed using the ECL technique.
Predicted band size: 32 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
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Immunoprecipitation - Anti-NRBF2 antibody (ab85733)Detection of Human NRBF2 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85733 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
