Anti-PGC1 alpha antibody (ab77210)
Key features and details
- Mouse monoclonal to PGC1 alpha
- Suitable for: WB, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2b
Overview
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Product name
Anti-PGC1 alpha antibody
See all PGC1 alpha primary antibodies -
Description
Mouse monoclonal to PGC1 alpha -
Host species
Mouse -
Specificity
The immunogen sequence for this antibody is 55% homologous with PGC1 beta (Uniprot: Q86YN6). -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment corresponding to Human PGC1 alpha aa 689-799.
Database link: Q9UBK2 -
Positive control
- PGC1 alpha transfected U2OS cell lysate
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General notes
This product was changed from ascites to tissue culture supernatant on 12/3/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 8% Sodium chloride, 0.6% Dibasic monohydrogen sodium phosphate, 0.2% Monobasic dihydrogen potassium phosphate, 0.2% Potassium chloride -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Molecular processes
- Mitochondrial transcription
Images
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Western blot - Anti-PGC1 alpha antibody (ab77210)Anti-PGC1 alpha antibody (ab77210) at 1 µg/ml + Recombinant fragment of human PGC1 alpha at 0.1 µg
Secondary
Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 91 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?Western blot against tagged recombinant protein immunogen using ab77210 antibody at 1/500 dilution. Predicted band size of immunogen is 92kDa.
This image was generated using the ascites version of the product.
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Flow Cytometry - Anti-PGC1 alpha antibody (ab77210)Overlay histogram showing HEK293 cells stained with ab77210 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77210, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
This image was generated using the ascites version of the product.
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Western blot - Anti-PGC1 alpha antibody (ab77210)Anti-PGC1 alpha antibody (ab77210) at 1 µg/ml + PGC1 alpha transfected U2OS cell lysate at 25 µg
Secondary
Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 91 kDa
Observed band size: 91 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.This image was generated using the ascites version of the product.
