Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ARID1A antibody [EPR13501-73] - BSA and Azide free (ab250617)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13501-73] to ARID1A - BSA and Azide free
  • Suitable for: IHC-P, ICC, Flow Cyt (Intra)
  • Reacts with: Human

Overview

  • Product name

    Anti-ARID1A antibody [EPR13501-73] - BSA and Azide free
    See all ARID1A primary antibodies

  • Description

    Rabbit monoclonal [EPR13501-73] to ARID1A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC, Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250617 is the carrier-free version of ab182561.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody [EPR13501-73] - BSA and Azide free (ab250617)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody [EPR13501-73] - BSA and Azide free (ab250617)
    This data was developed using ab182561, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue (left image) labeling ARID1A using ab182561 at 1/1000 dilution. Right image: human clear cell carcinoma of kidney with ARID1A mutation. A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-ARID1A antibody [EPR13501-73] - BSA and Azide free (ab250617)
    Immunocytochemistry - Anti-ARID1A antibody [EPR13501-73] - BSA and Azide free (ab250617)
    This data was developed using ab182561, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of SH-SY5Y cells labeling ARID1A with ab182561 at 1/500 and Goat anti rabbit IgG(Alexa Fluor®555) at 1/200. Image at the right stained with DAPI.
  • Flow Cytometry - Anti-ARID1A antibody [EPR13501-73] - BSA and Azide free (ab250617)
    Flow Cytometry - Anti-ARID1A antibody [EPR13501-73] - BSA and Azide free (ab250617)
    This data was developed using ab182561, the same antibody clone in a different buffer formulation.Flow Cytometry analysis of SH-SY5Y (human neuroblastoma) cells labeling ARID1A with purified ab92512 at 1/500 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody [EPR13501-73] - BSA and Azide free (ab250617)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody [EPR13501-73] - BSA and Azide free (ab250617)
    This data was developed using ab182561, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human adenocarcinoma of endometrium without ARID1A mutation (left image) labeling ARID1A using ab182561 at 1/1000 dilution. Right image: human adenocarcinoma of endometrium with ARID1A mutation. A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Anti-ARID1A antibody [EPR13501-73] - BSA and Azide free (ab250617)
    Anti-ARID1A antibody [EPR13501-73] - BSA and Azide free (ab250617)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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