Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human FOXO1A Antibody Pair - BSA and Azide free (ab241850)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Human

Overview

  • Product name

    Human FOXO1A Antibody Pair - BSA and Azide free
    See all FOXO1A kits

  • Assay type

    ELISA set

  • Range

    125 pg/ml - 8000 pg/ml

  • Species reactivity

    Reacts with: Human

  • Product overview

    The Antibody Pair can be used to quantify Human FOXO1A. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab215087), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    Download SDS here.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.

  • Carrier free

    Yes
  • Components 10 x 96 tests
    Human FOXO1A Capture Antibody (unconjugated) 1 x 100µg
    Human FOXO1A Detector Antibody (unconjugated) 1 x 100µg

  • Research areas

  • Function

    Transcription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence.

  • Tissue specificity

    Ubiquitous.

  • Involvement in disease

    Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=Chromosomal aberrations involving FOXO1 are found in rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.

  • Sequence similarities

    Contains 1 fork-head DNA-binding domain.

  • Post-translational
    modifications

    Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
  • Information by UniProt

  • Alternative names

    • FKH 1
    • FKH1
    • FKHR
    • Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma)
    • Forkhead box O1
    • Forkhead box protein O1
    • Forkhead box protein O1A
    • Forkhead in rhabdomyosarcoma
    • Forkhead, Drosophila, homolog of, in rhabdomyosarcoma
    • FoxO transcription factor
    • foxo1
    • FOXO1_HUMAN
    • FOXO1A
    • OTTHUMP00000018301
    see all

  • Database links

    Images

    • Sandwich ELISA - Human FOXO1A Antibody Pair - BSA and Azide free (ab241850)
      Sandwich ELISA - Human FOXO1A Antibody Pair - BSA and Azide free (ab241850)
      To learn more about the advantages of recombinant antibodies see here.

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