Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Tuesday March 02, 2021

Anti-XRCC1 antibody [EPR4389(2)] (ab134056)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4389(2)] to XRCC1
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-XRCC1 antibody [EPR4389(2)]
See all XRCC1 primary antibodies
Description

Rabbit monoclonal [EPR4389(2)] to XRCC1
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Mouse Rat Human
IHC-P	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human XRCC1 aa 1-100 (N terminal). The exact sequence is proprietary.
Positive control
- WB: HeLa, A375, Saos-2, PC-12 and NIH/3T3 cell lysates. Mouse brain and kidney tissue lysate IHC-P: Human testis tissue. ICC/IF: HeLa, PC-12 and NIH/3T3 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR4389(2)
Isotype

IgG
Research areas

Western blot - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)

All lanes : Anti-XRCC1 antibody [EPR4389(2)] (ab134056) at 1/2000 dilution (purified)

Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse kidney lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 4 : NIH/3T3(Mouse embryonic fibroblast) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated(Pierce) at 1/1000 dilution

Predicted band size: 69 kDa
Observed band size: 85 kDa
why is the actual band size different from the predicted?

Blocking/Diluting buffer: 5% NFDM /TBST

Exposure time:
Lanes 1-2: 3 minutes
Lanes 3-4: 20 seconds
Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)

Immunocytochemistry/Immunofluorescence analysis of PC-12(Rat adrenal gland pheochromocytoma) labelling with ab134056 at a dilution of 1/50 dilution (4.66 µg/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) (1/1000 dilution (2 µg/mL)) was used as the secondary antibody. The cells were co-stained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 µg/mL). Nuclei counterstained with DAPI (blue). Control: PBS instead of the primary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)

Immunocytochemistry/Immunofluorescence analysis of NIH/3T3(Mouse embryonic fibroblast) labelling with ab134056 at a dilution of 1/50 (4.66 µg/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/1000 dilution (2 µg/mL)) was used as the secondary antibody. The cells were co-stained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 µg/mL). Nuclei counterstained with DAPI (blue). Control: PBS instead of the primary antibody.
Western blot - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)

All lanes : Anti-XRCC1 antibody [EPR4389(2)] (ab134056) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : A375 cell lysate
Lane 3 : Saos-2 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 69 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling XRCC1 with ab134056 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (ab134056) This image is courtesy of an Abreview submitted by Kirk McManus

ab134056 staining XRCC1 in human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Counterstained with DAPI.
See Abreview
Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)

Immunofluorescent analysis of HeLa cells labelling XRCC1 with ab134056 at 1/100 dilution.
Anti-XRCC1 antibody [EPR4389(2)] (ab134056)

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