Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-XRCC1 antibody [EPR4389(2)] (ab134056)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4389(2)] to XRCC1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-XRCC1 antibody [EPR4389(2)]
    See all XRCC1 primary antibodies

  • Description

    Rabbit monoclonal [EPR4389(2)] to XRCC1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    Rat
    Human
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human XRCC1 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HeLa, A375, Saos-2, PC-12 and NIH/3T3 cell lysates. Mouse brain and kidney tissue lysate IHC-P: Human testis tissue. ICC/IF: HeLa, PC-12 and NIH/3T3 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)
    Western blot - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)
    All lanes : Anti-XRCC1 antibody [EPR4389(2)] (ab134056) at 1/2000 dilution (purified)

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 4 : NIH/3T3(Mouse embryonic fibroblast) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated(Pierce) at 1/1000 dilution

    Predicted band size: 69 kDa
    Observed band size: 85 kDa
    why is the actual band size different from the predicted?



    Blocking/Diluting buffer: 5% NFDM /TBST

    Exposure time:
    Lanes 1-2: 3 minutes
    Lanes 3-4: 20 seconds

  • Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)
    Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)

     Immunocytochemistry/Immunofluorescence analysis of PC-12(Rat adrenal gland pheochromocytoma) labelling with ab134056 at a dilution of 1/50 dilution (4.66 µg/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) (1/1000 dilution (2 µg/mL)) was used as the secondary antibody. The cells were co-stained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Nuclei counterstained with DAPI (blue). Control: PBS instead of the primary antibody.


     

  • Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)
    Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)

    Immunocytochemistry/Immunofluorescence analysis of NIH/3T3(Mouse embryonic fibroblast) labelling with ab134056 at a dilution of 1/50 (4.66 µg/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 dilution (2 µg/mL)) was used as the secondary antibody. The cells were co-stained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Nuclei counterstained with DAPI (blue). Control: PBS instead of the primary antibody.

  • Western blot - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)
    Western blot - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)
    All lanes : Anti-XRCC1 antibody [EPR4389(2)] (ab134056) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A375 cell lysate
    Lane 3 : Saos-2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 69 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling XRCC1 with ab134056 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)
    Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (ab134056) This image is courtesy of an Abreview submitted by Kirk McManus

    ab134056 staining XRCC1 in human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Counterstained with DAPI.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)
    Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] (ab134056)
    Immunofluorescent analysis of HeLa cells labelling XRCC1 with ab134056 at 1/100 dilution.
  • Anti-XRCC1 antibody [EPR4389(2)] (ab134056)
    Anti-XRCC1 antibody [EPR4389(2)] (ab134056)

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