Anti-POLRMT antibody (ab32988)
Key features and details
- Rabbit polyclonal to POLRMT
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-POLRMT antibody
See all POLRMT primary antibodies -
Description
Rabbit polyclonal to POLRMT -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human POLRMT aa 1200 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab32987) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates; HeLa, HEK293 and Caco2.
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General notes
This product was previously labelled as mtRNA polymerase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32988 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseApplication Abreviews Notes WB 1/250. Detects a band of approximately 140 kDa (predicted molecular weight: 139 kDa).Notes WB
1/250. Detects a band of approximately 140 kDa (predicted molecular weight: 139 kDa).Target
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Function
DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates. -
Sequence similarities
Belongs to the phage and mitochondrial RNA polymerase family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 5442 Human
- Entrez Gene: 216151 Mouse
- Omim: 601778 Human
- SwissProt: O00411 Human
- SwissProt: Q8BKF1 Mouse
- Unigene: 254113 Human
- Unigene: 39001 Mouse
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Alternative names
- APOLMT antibody
- DNA-directed RNA polymerase antibody
- DNA-directed RNA polymerase mitochondrial antibody
see all
Images
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Western blot - Anti-POLRMT antibody (ab32988)All lanes : Anti-POLRMT antibody (ab32988) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 139 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Additional bands at: 160 kDa, 45 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
References (8)
ab32988 has been referenced in 8 publications.
- Zhu Z et al. Negative effects of ROS generated during linear sperm motility on gene expression and ATP generation in boar sperm mitochondria. Free Radic Biol Med 141:159-171 (2019). PubMed: 31212063
- van Esveld SL et al. A Combined Mass Spectrometry and Data Integration Approach to Predict the Mitochondrial Poly(A) RNA Interacting Proteome. Front Cell Dev Biol 7:283 (2019). PubMed: 31803741
- Herbers E et al. Tissue specific differences in mitochondrial DNA maintenance and expression. Mitochondrion N/A:N/A (2018). WB . PubMed: 29339192
- Nouws J et al. Mitochondrial Ribosomal Protein L12 Is Required for POLRMT Stability and Exists as Two Forms Generated by Alternative Proteolysis during Import. J Biol Chem 291:989-97 (2016). PubMed: 26586915
- Bralha FN et al. Targeting mitochondrial RNA polymerase in acute myeloid leukemia. Oncotarget 6:37216-28 (2015). WB . PubMed: 26484416
- Harmel J et al. The leucine-rich pentatricopeptide repeat-containing protein (LRPPRC) does not activate transcription in mammalian mitochondria. J Biol Chem 288:15510-9 (2013). WB ; Mouse . PubMed: 23599432
- Sobek S et al. Negative regulation of mitochondrial transcription by mitochondrial topoisomerase I. Nucleic Acids Res N/A:N/A (2013). PubMed: 23982517
- Taha R et al. Oxidative stress and mitochondrial functions in the intestinal Caco-2/15 cell line. PLoS One 5:e11817 (2010). WB ; Human . PubMed: 20676402
Images
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Western blot - Anti-POLRMT antibody (ab32988)All lanes : Anti-POLRMT antibody (ab32988) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 139 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Additional bands at: 160 kDa, 45 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
