Anti-RNA polymerase II CTD repeat YSPTSPS antibody (ab52202)
Key features and details
- Rabbit polyclonal to RNA polymerase II CTD repeat YSPTSPS
- Suitable for: WB, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-RNA polymerase II CTD repeat YSPTSPS antibody
See all RNA polymerase II CTD repeat YSPTSPS primary antibodies -
Description
Rabbit polyclonal to RNA polymerase II CTD repeat YSPTSPS -
Host species
Rabbit -
Specificity
ab52202 detects endogenous levels of total RNA polymerase II protein. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB HumanAfrican green monkey
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Immunogen
Synthetic peptide corresponding to Human RNA polymerase II CTD repeat YSPTSPS aa 1585-1634. Immunogen is in the range of aa 1585 and 1634.
Database link: P24928 -
Positive control
- WB: HepG2, HeLa, Hek293, SW480 cell lysate; COS7 cells treated with EGF (200ng/ml, 30min). IHC-P: Human breast carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS antibody (ab52202)Lanes 1-3 : Anti-RNA polymerase II CTD repeat YSPTSPS antibody (ab52202) at 1/1000 dilution
Lane 4 : Anti-Swr1 antibody (ab5202) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Hek293 cell lysate
Lane 4 : SW480 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (H+L) HRP at 1/10000 dilution
Predicted band size: 217 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNA polymerase II CTD repeat YSPTSPS antibody (ab52202)Immunohistochemical analysis of RNA polymerase II expression in paraffin embedded human breast carcinoma tissue using ab52202 at 1/500.
Left: sample without immunising peptide.
Right: sample with immunising peptide (negative control). -
Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS antibody (ab52202)All lanes : Anti-RNA polymerase II CTD repeat YSPTSPS antibody (ab52202) at 1/500 dilution
Lane 1 : COS7 cells treated
with EGF (200ng/ml, 30min), without immunising peptide
Lane 2 : COS7 cells treated
with EGF (200ng/ml, 30min), with immunising peptide
Predicted band size: 217 kDa
Observed band size: 217 kDa
