Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PRAK/MK5 (phospho T182) antibody (ab138668)

Key features and details

  • Rabbit polyclonal to PRAK/MK5 (phospho T182)
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-PRAK/MK5 (phospho T182) antibody
    See all PRAK/MK5 primary antibodies

  • Description

    Rabbit polyclonal to PRAK/MK5 (phospho T182)

  • Host species

    Rabbit

  • Specificity

    ab138668 detects endogenous levels of PRAK/MK5 only when phosphorylated at T182.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human PRAK/MK5 (internal sequence).

  • Positive control

    • WB: K562 cell lysate from cells treated with sodium orthovanadate. IHC-P: Human breast carcinoma tissue.

  • General notes

     This product was previously labelled as PRAK

     

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    PBS is without Mg2+ and Ca2+
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    ab138668 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRAK/MK5 (phospho T182) antibody (ab138668)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRAK/MK5 (phospho T182) antibody (ab138668)

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue stained for PRAK/MK5 (phospho T182) using ab138668 at 1/50 dilution. The right panel shows the effect of blocking with the immunozing phospho-peptide.

  • Western blot - Anti-PRAK/MK5 (phospho T182) antibody (ab138668)
    Western blot - Anti-PRAK/MK5 (phospho T182) antibody (ab138668)
    Anti-PRAK/MK5 (phospho T182) antibody (ab138668) at 1/500 dilution + K562 cell lysate (from cells treated with 0.3 nM sodium orthovanadate for 40 minutes) at 30 µg

    Predicted band size: 54 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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