Anti-PRAK/MK5 (phospho T182) antibody (ab138668)
Key features and details
- Rabbit polyclonal to PRAK/MK5 (phospho T182)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PRAK/MK5 (phospho T182) antibody
See all PRAK/MK5 primary antibodies -
Description
Rabbit polyclonal to PRAK/MK5 (phospho T182) -
Host species
Rabbit -
Specificity
ab138668 detects endogenous levels of PRAK/MK5 only when phosphorylated at T182.
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Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human PRAK/MK5 (internal sequence).
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Positive control
- WB: K562 cell lysate from cells treated with sodium orthovanadate. IHC-P: Human breast carcinoma tissue.
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General notes
This product was previously labelled as PRAK
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab138668 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRAK/MK5 (phospho T182) antibody (ab138668)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue stained for PRAK/MK5 (phospho T182) using ab138668 at 1/50 dilution. The right panel shows the effect of blocking with the immunozing phospho-peptide.
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Western blot - Anti-PRAK/MK5 (phospho T182) antibody (ab138668)Anti-PRAK/MK5 (phospho T182) antibody (ab138668) at 1/500 dilution + K562 cell lysate (from cells treated with 0.3 nM sodium orthovanadate for 40 minutes) at 30 µg
Predicted band size: 54 kDa
