Human TIA1 ELISA Kit (ab231921)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 117 pg/ml
- Range: 188 pg/ml - 12000 pg/ml
- Sample type: Cell culture extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human TIA1 ELISA Kit
See all TIA1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Cell extract 5 6.8% Inter-assay Sample n Mean SD CV% Cell extract 3 4.8% -
Sample type
Cell culture extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
117 pg/ml -
Range
188 pg/ml - 12000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 109 105% - 114% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human TIA1 ELISA Kit (ab231921) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TIA1 protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TIA1 with 117 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human TIA1 Capture Antibody 1 x 600µl 10X Human TIA1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Human TIA1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis. -
Sequence similarities
Contains 3 RRM (RNA recognition motif) domains. -
Cellular localization
Cytoplasmic granule. Nucleus. Accumulates in cytoplasmic stress granules (SG) following cellular damage. - Information by UniProt
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Alternative names
- Cytotoxic granule associated RNA binding protein
- Cytotoxic granule associated RNA binding protein 1
- mTIA-1
see all -
Database links
- Entrez Gene: 7072 Human
- Omim: 603518 Human
- SwissProt: P31483 Human
- Unigene: 413123 Human
Images
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Other - Human TIA1 ELISA Kit (ab231921)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human TIA1 standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native TIA1 in human Jurkat, HUVEC, HepG2, and MOLT-4 cell extract samples based on 250 µg/mL, 500 µg/mL, 500 µg/mL, and 200 µg/mL extract loads, respectively.The concentrations of TIA1 were measured in duplicate and interpolated from the TIA1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIA1 concentration was determined to be 11,185 pg/mL in Jurkat, 10,313 pg/ml in HUVEC, 9394 pg/ml in HepG2, and 5,642 pg/mL in MOLT-4 cell extract samples.
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Sandwich ELISA - Human TIA1 ELISA Kit (ab231921)To learn more about the advantages of recombinant antibodies see here.