Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Estrogen Receptor alpha antibody [CRET94D] (ab241557)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Mouse monoclonal [CRET94D] to Estrogen Receptor alpha
Suitable for: IHC-P, WB
Reacts with: Mouse, Human

Product name

Anti-Estrogen Receptor alpha antibody [CRET94D]
See all Estrogen Receptor alpha primary antibodies
Description

Mouse monoclonal [CRET94D] to Estrogen Receptor alpha
Host species

Mouse
Tested Applications & Species

Application Species
IHC-P
Mouse

Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human breast cancer tissue, mouse breast tissue and mouse ovary tissue lysates. IHC: Human breast carcinoma and mouse breast tissue.
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

This product has switched from a hybridoma to recombinant production method on 07^th December 2020.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Application	Species
IHC-P	Mouse Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

CRET94D
Isotype

IgG1
Light chain type

kappa
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [CRET94D] (ab241557)

Immunohistochemical analysis of paraffin-embedded mouse breast labeling Estrogen Receptor with ab252794 at 1/1000 dilution followed by LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab252794 for 30 mins at room temperature and blocking mouse IgG by specific antibody (ab133469, 1:5000) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylinFor other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [CRET94D] (ab241557)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma and mouse breast tissues labeling Estrogen Receptor with ab252794 at 1/1000 dilution followed by LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab252794 for 30 mins at room temperature and blocking mouse IgG by specific antibody (ab133469, 1:5000) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylinFor other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Anti-Estrogen Receptor alpha antibody [CRET94D] (ab241557)

All lanes : Anti-Estrogen Receptor alpha antibody [CRET94D] (ab241557) at 1/1000 dilution

Lane 1 : Human breast cancer tissue lysates
Lane 2 : Mouse breast tissue lysates
Lane 3 : Mouse ovary tissue lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-mouse IgG for IP (HRP) (ab131368)

Predicted band size: 66 kDa
Observed band size: 66 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com