Phospho-Estrogen receptor alpha (S118) and Total Estrogen receptor alpha (ab279787) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human and cell lysates. By determining phosphorylated Estrogen receptor alpha protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-Estrogen receptor alpha (S118) and total Estrogen receptor alpha. An anti-pan Estrogen receptor alpha antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Estrogen receptor alpha present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho-ER-alpha (S118) antibody is added to detect phosphorylated Estrogen receptor alpha. In the remaining wells, mouse anti-pan-ER alpha antibody is used to detect pan Estrogen receptor alpha. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-conjugated anti-mouse IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Estrogen receptor alpha (S118) or pan Estrogen receptor alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

• Signal Transduction
• Signaling Pathway
• Nuclear Signaling
• Nuclear Hormone Receptors
• Estrogen

• Neuroscience
• Endocrine system
• Gonadotrophic axis

• Epigenetics and Nuclear Signaling
• Nuclear Signaling Pathways
• Nuclear Receptors
• Estrogen

• Cancer
• Signal transduction
• Nuclear signaling
• Nuclear hormone receptors
• Estrogen