Anti-Histone H3 (methyl K37) antibody [EPR20970] - BSA and Azide free (ab236275)
![Anti-Histone H3 (methyl K37) antibody [EPR20970] - BSA and Azide free (ab236275)](https://www.abcam.com/ps/products/236/ab236275/Images/ab236275-414817-ab2157281215728WB1.jpg "Anti-Histone H3 (methyl K37) antibody [EPR20970] - BSA and Azide free (ab236275)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20970] to Histone H3 (methyl K37) - BSA and Azide free
- Suitable for: WB, Flow Cyt, ICC, PepArr
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Overview
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Product name
Anti-Histone H3 (methyl K37) antibody [EPR20970] - BSA and Azide free
See all Histone H3 primary antibodies -
Description
Rabbit monoclonal [EPR20970] to Histone H3 (methyl K37) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, ICC, PepArrmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Recombinant fragment -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Recombinant mutant H3 chemically modified to mimic H3K37Me; NCCIT, HeLa, NIH/3T3 and PC-12 whole cell lysates. ICC: HeLa and NIH/3T3 cells. Flow cyt: HeLa and NIH/3T3 cells.
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General notes
ab236275 is the carrier-free version of ab215728. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab236275 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20970 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Histone H3 (methyl K37) antibody [EPR20970] - BSA and Azide free (ab236275)All lanes : Anti-Histone H3 (methyl K37) antibody [EPR20970] (ab215728) at 1/1000 dilution
Lane 1 : Unmodified recombinant H3 10 ng
Lane 2 : Recombinant mutant H3 chemically modified to mimic H3K37Me, 10ng
Lane 3 : Recombinant mutant H3 chemically modified to mimic H3K37Me2 10 ng
Lane 4 : Recombinant mutant H3 chemically modified to mimic H3K37Me3 10 ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 15 kDaThis data was developed using ab215728, the same antibody clone in a different buffer formulation.
Blocking/Dilution buffer: 5% NFDM/TBST.
The recombinant proteins were His-tagged.
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![Immunocytochemistry - Anti-Histone H3 (methyl K37) antibody [EPR20970] - BSA and Azide free (ab236275)](https://www.abcam.com/ps/products/236/ab236275/Images/ab236275-414816-ab2157284215728ICC2.jpg)
Immunocytochemistry - Anti-Histone H3 (methyl K37) antibody [EPR20970] - BSA and Azide free (ab236275)This data was developed using ab215728, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryonic fibroblast cell line) cells labeling Histone H3 (methyl K37) with ab215728 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining in NIH/3T3 cell line is observed. DAPI was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used as the counterstain antibody at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
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![Flow Cytometry - Anti-Histone H3 (methyl K37) antibody [EPR20970] - BSA and Azide free (ab236275)](https://www.abcam.com/ps/products/236/ab236275/Images/ab236275-414819-ab2157286215728FC2.jpg)
Flow Cytometry - Anti-Histone H3 (methyl K37) antibody [EPR20970] - BSA and Azide free (ab236275)This data was developed using ab215728, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 90% methanol permeabilized, 4% paraformaldehyde-fixed NIH/3T3 (mouse embryo fibroblast cell line) cells labeling Histone H3 (methyl K37) with ab215728 at 1/60 dilution (Red) compared with Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.
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![Western blot - Anti-Histone H3 (methyl K37) antibody [EPR20970] - BSA and Azide free (ab236275)](https://www.abcam.com/ps/products/236/ab236275/Images/ab236275-414818-ab2157282215728WB2.jpg)
Western blot - Anti-Histone H3 (methyl K37) antibody [EPR20970] - BSA and Azide free (ab236275)All lanes : Anti-Histone H3 (methyl K37) antibody [EPR20970] (ab215728) at 1/1000 dilution
Lane 1 : NCCIT (human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 15 kDaThis data was developed using ab215728, the same antibody clone in a different buffer formulation.
Exposure times:
Lane 1: 26 seconds;
Lane 2: 3 minutes;
Lane 3: 81 seconds;
Lane 4: 3 minutes.Blocking/Dilution buffer: 5% BSA/TBST
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![Immunocytochemistry - Anti-Histone H3 (methyl K37) antibody [EPR20970] - BSA and Azide free (ab236275)](https://www.abcam.com/ps/products/236/ab236275/Images/ab236275-312032-84.jpg)
Immunocytochemistry - Anti-Histone H3 (methyl K37) antibody [EPR20970] - BSA and Azide free (ab236275)This data was developed using ab215728, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell line) cells labeling Histone H3 (methyl K37) with ab215728 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining in HeLa cell line is observed. DAPI was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used as the counterstain antibody at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
-
![Flow Cytometry - Anti-Histone H3 (methyl K37) antibody [EPR20970] - BSA and Azide free (ab236275)](https://www.abcam.com/ps/products/236/ab236275/Images/ab236275-414820-ab2157285215728FC1.jpg)
Flow Cytometry - Anti-Histone H3 (methyl K37) antibody [EPR20970] - BSA and Azide free (ab236275)This data was developed using ab215728, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 90% methanol permeabilized, 4% paraformaldehyde-fixed HeLa (human cervix adenocarcinoma epithelial cell) cells labeling Histone H3 (methyl K37) with ab215728 at 1/60 dilution (Red) compared with Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.
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![Peptide Array - Anti-Histone H3 (methyl K37) antibody [EPR20970] - BSA and Azide free (ab236275)](https://www.abcam.com/ps/products/236/ab236275/Images/ab236275-414821-ab215728312124215728666.jpg)
Peptide Array - Anti-Histone H3 (methyl K37) antibody [EPR20970] - BSA and Azide free (ab236275)This data was developed using ab215728, the same antibody clone in a different buffer formulation.
ab215728 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Blocking buffer: 5% BSA in TBST.
Primary antibody concentration: 0.1 µg/ml.
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.