Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H2A (symmetric di methyl R11) antibody [E18766] (ab195464)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E18766] to Histone H2A (symmetric di methyl R11)
  • Suitable for: WB, PepArr
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Histone H2A (symmetric di methyl R11) antibody [E18766]
    See all Histone H2A primary antibodies

  • Description

    Rabbit monoclonal [E18766] to Histone H2A (symmetric di methyl R11)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    PepArr
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human ovary; NT2/D1, HL60, NCCIT, F9, mESC, mouse ovary lysate, C2C12, 3T3-L1, HeLa and NIH 3T3 lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    Production method: Hybridoma.

  • Clonality

    Monoclonal

  • Clone number

    E18766

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Histone H2A (symmetric di methyl R11) antibody [E18766] (ab195464)
    Western blot - Anti-Histone H2A (symmetric di methyl R11) antibody [E18766] (ab195464)
    All lanes : Anti-Histone H2A (symmetric di methyl R11) antibody [E18766] (ab195464) at 1/500 dilution

    Lane 1 : Human ovary lysate
    Lane 2 : NT2/D1(human malignant pluripotent embryonic carcinoma ) whole cell lysate
    Lane 3 : HL60 (Human promyelocytic leukemia cells) whole cell lysate
    Lane 4 : NCCIT(human pluripotent embryonic carcinoma) whole cell lysate
    Lane 5 : F9 (Mouse embryo testicular cancer cell line) whole cell lysate
    Lane 6 : Human placenta lysate
    Lane 7 : mESC (mouse embryo stem cell) whole cell lysate
    Lane 8 : Mouse ovary lysate
    Lane 9 : C2C12 (Mouse myoblast cell line) whole cell lysate
    Lane 10 : 3T3-L1 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 11 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 12 : NIH 3T3 (Mouse embryo fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

    Predicted band size: 14 kDa


    Exposure time: 3 minutes


    5% NFDM/TBST: Blocking and diluting buffer.

  • Peptide Array - Anti-Histone H2A (symmetric di methyl R11) antibody [E18766] (ab195464)
    Peptide Array - Anti-Histone H2A (symmetric di methyl R11) antibody [E18766] (ab195464)

    ab195464 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
    The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.

  • Anti-Histone H2A (symmetric di methyl R11) antibody [E18766] (ab195464)
    Anti-Histone H2A (symmetric di methyl R11) antibody [E18766] (ab195464)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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