Anti-Histone H2A (symmetric di methyl R11) antibody [E18766] (ab195464)
![Anti-Histone H2A (symmetric di methyl R11) antibody [E18766] (ab195464)](https://www.abcam.com/ps/products/195/ab195464/Images/ab195464-249760-anti-histone-h2a-symmetric-di-methyl-r11-antibody-e18766-western-blot.jpg "Anti-Histone H2A (symmetric di methyl R11) antibody [E18766] (ab195464)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E18766] to Histone H2A (symmetric di methyl R11)
- Suitable for: WB, PepArr
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Histone H2A (symmetric di methyl R11) antibody [E18766]
See all Histone H2A primary antibodies -
Description
Rabbit monoclonal [E18766] to Histone H2A (symmetric di methyl R11) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species PepArr HumanWB MouseHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human ovary; NT2/D1, HL60, NCCIT, F9, mESC, mouse ovary lysate, C2C12, 3T3-L1, HeLa and NIH 3T3 lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Production method: Hybridoma. -
Clonality
Monoclonal -
Clone number
E18766 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Histone H2A (symmetric di methyl R11) antibody [E18766] (ab195464)All lanes : Anti-Histone H2A (symmetric di methyl R11) antibody [E18766] (ab195464) at 1/500 dilution
Lane 1 : Human ovary lysate
Lane 2 : NT2/D1(human malignant pluripotent embryonic carcinoma ) whole cell lysate
Lane 3 : HL60 (Human promyelocytic leukemia cells) whole cell lysate
Lane 4 : NCCIT(human pluripotent embryonic carcinoma) whole cell lysate
Lane 5 : F9 (Mouse embryo testicular cancer cell line) whole cell lysate
Lane 6 : Human placenta lysate
Lane 7 : mESC (mouse embryo stem cell) whole cell lysate
Lane 8 : Mouse ovary lysate
Lane 9 : C2C12 (Mouse myoblast cell line) whole cell lysate
Lane 10 : 3T3-L1 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 11 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 12 : NIH 3T3 (Mouse embryo fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size: 14 kDa
Exposure time: 3 minutes5% NFDM/TBST: Blocking and diluting buffer.
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![Peptide Array - Anti-Histone H2A (symmetric di methyl R11) antibody [E18766] (ab195464)](https://www.abcam.com/ps/products/195/ab195464/Images/ab195464-249761-anti-histone-h2a-symmetric-di-methyl-r11-antibody-e18766-peptide-array.jpg)
Peptide Array - Anti-Histone H2A (symmetric di methyl R11) antibody [E18766] (ab195464)ab195464 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here. -
![Anti-Histone H2A (symmetric di methyl R11) antibody [E18766] (ab195464)](https://www.abcam.com/ps/products/195/ab195464/Images/ab195464-5-benefits-of-recombinant-antibodies.png)
Anti-Histone H2A (symmetric di methyl R11) antibody [E18766] (ab195464)
