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Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [106-6] to TMEM119 - Microglial marker
  • Suitable for: Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-TMEM119 antibody [106-6] - Microglial marker
    See all TMEM119 primary antibodies

  • Description

    Rabbit monoclonal [106-6] to TMEM119 - Microglial marker

  • Host species

    Rabbit

  • Specificity

    Human reactivity has not been tested.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cytometry: Acutely isolated primary mouse microglia (P60 BL6 mouse; wildtype CD11b+CD45lo brain cells), HEK-293T transfected with Myc-His tagged TMEM119

  • General notes

    Please note, this antibody is suitable for flow cytometry. For IHC on mouse brain tissue we recommend ab209064.

    Please note that the original Bennett et al. (2016) publication (PubMed: 26884166), used a combination of clones 106-6 and 85-5. With the author’s permission, the decision was made to add the recombinant version of only a single clone (106-6) to the catalogue as it performed equally well on its own.

    The 106-6 clone to mouse Tmem119 is exclusively manufactured and sold by Abcam.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    106-6

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405)
    Flow Cytometry - Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405)

    Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged TMEM119 expression vector labeling TMEM119 with ab210405 at 1/2000 dilution (0.1μg/mL) (right) compared with isotype control rabbit monoclonal IgG ab172730 (Left). Cells were surface-stained with ab210405, then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 647 conjugated Myc-tag antibody and Alexa Fluor® 488 conjugated secondary antibody. Only Myc-tag (+) population showed TMEM119 positive staining.

    Alexa Fluor® 488 (ab225497) and R-PE (ab225496) conjugated versions are available for this clone.

  • Flow Cytometry - Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405)
    Flow Cytometry - Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405)

    Flow cytometric analysis of acutely isolated primary mouse microglia (P60 BL6 mouse; wildtype CD11b+CD45lo brain cells) cells  labeling TMEM119 with ab210405 at 0.5μg/mL (red) and 0.1μg/mL (blue), compared with TMEM119 KO primary mouse brain cells (black) stained with ab210405 at 0.5μg/mL. Goat anti-Rabbit IgG (Alexa Fluor®488) at 1/500 dilution was used as the secondary antibody.

    No signal was detected on the surface of CD11b+CD45lo brain cells from TMEM119 KO mouse (black) stained with ab210405; whereas in wildtype CD11b+CD45lo brain cells, cell surface staining was observed (red 0.5ug/mL; blue 0.1ug/mL).

    The data was provided by Ben Barres’ lab (Stanford University).

  • Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405)
    Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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