Anti-Annexin A1/ANXA1 antibody [EPR2767(2)] (ab138512)
![Anti-Annexin A1/ANXA1 antibody [EPR2767(2)] (ab138512)](https://www.abcam.com/ps/products/138/ab138512/Images/ab138512-278163-anti-annexin-i-antibody-epr27672-western-blot.jpg "Anti-Annexin A1/ANXA1 antibody [EPR2767(2)] (ab138512)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2767(2)] to Annexin A1/ANXA1
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Annexin A1/ANXA1 antibody [EPR2767(2)]
See all Annexin A1/ANXA1 primary antibodies -
Description
Rabbit monoclonal [EPR2767(2)] to Annexin A1/ANXA1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB RatHuman
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Immunogen
Synthetic peptide within Human Annexin A1/ANXA1 aa 300 to the C-terminus. The exact sequence is proprietary.
Database link: P04083 -
Positive control
- C6, Raw264.7, NIH3T3, C2C12, K562, A431, and HeLa whole cell lysate (ab150035)
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2767(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Annexin A1/ANXA1 antibody [EPR2767(2)] (ab138512)
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Annexin I knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)Lanes 1 - 3: Merged signal (red and green). Green - ab138512 observed at 40 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab138512 was shown to specifically react with Annexin I when Annexin I knockout samples were used. Wild-type and Annexin I knockout samples were subjected to SDS-PAGE. Ab138512 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Annexin A1/ANXA1 antibody [EPR2767(2)] (ab138512)](https://www.abcam.com/ps/products/138/ab138512/Images/ab138512-153801-anti-annexin-i-antibody-epr27672-western-blot.jpg)
Western blot - Anti-Annexin A1/ANXA1 antibody [EPR2767(2)] (ab138512)All lanes : Anti-Annexin A1/ANXA1 antibody [EPR2767(2)] (ab138512) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : Raw264.7 cell lysate
Lane 3 : NIH3T3 cell lysate
Lane 4 : C2C12 cell lysate
Lane 5 : K562 cell lysate
Lane 6 : A431 cell lysate
Lane 7 : HeLa cell lysate
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 39 kDa
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![Anti-Annexin A1/ANXA1 antibody [EPR2767(2)] (ab138512)](https://www.abcam.com/ps/products/138/ab138512/Images/ab138512-3-benefits-of-recombinant-antibodies.png)
Anti-Annexin A1/ANXA1 antibody [EPR2767(2)] (ab138512)