Anti-AG-3 antibody [EPR19606] - BSA and Azide free (ab251346)
![Anti-AG-3 antibody [EPR19606] - BSA and Azide free (ab251346)](https://www.abcam.com/ps/products/251/ab251346/Images/ab251346-405920-anti-ag-3-antibody-epr19606-bsa-and-azide-free-western-blot.jpg "Anti-AG-3 antibody [EPR19606] - BSA and Azide free (ab251346)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19606] to AG-3 - BSA and Azide free
- Suitable for: Flow Cyt, ICC, WB
- Reacts with: Human
Overview
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Product name
Anti-AG-3 antibody [EPR19606] - BSA and Azide free
See all AG-3 primary antibodies -
Description
Rabbit monoclonal [EPR19606] to AG-3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, ICC, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251346 is the carrier-free version of ab201464. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251346 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR19606 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AG-3 antibody [EPR19606] - BSA and Azide free (ab251346)Lane 1 : Anti-AG-3 antibody [EPR19606] (ab201464) at 1/2000 dilution
Lane 2 : Anti-AG-3 antibody [EPR19606] (ab201464) at 1/10000 dilution
Lanes 3-5 : Anti-AG-3 antibody [EPR19606] (ab201464) at 1/1000 dilution
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate
Lane 3 : Human colon cancer tissue lysate
Lane 4 : Human breast cancer tissue lysate
Lane 5 : Human ovary cancer tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 3-5 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDaThis data was developed using ab201464, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1 and 5: 15 seconds; Lanes 2-4: 3 minutes.
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![Immunocytochemistry - Anti-AG-3 antibody [EPR19606] - BSA and Azide free (ab251346)](https://www.abcam.com/ps/products/251/ab251346/Images/ab251346-2-anti-ag-3-antibody-epr19606-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-AG-3 antibody [EPR19606] - BSA and Azide free (ab251346)This data was developed using ab201464, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling AG-3 with ab201464 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on MCF7 cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red). The negative controls are as follows:
-ve control 1: ab201464 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution. -
![Flow Cytometry - Anti-AG-3 antibody [EPR19606] - BSA and Azide free (ab251346)](https://www.abcam.com/ps/products/251/ab251346/Images/ab251346-1-anti-ag-3-antibody-epr19606-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-AG-3 antibody [EPR19606] - BSA and Azide free (ab251346)This data was developed using ab201464, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling AG-3 with ab201464 at 1/600 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. -
![Anti-AG-3 antibody [EPR19606] - BSA and Azide free (ab251346)](https://www.abcam.com/ps/products/251/ab251346/Images/ab251346-4-benefits-of-recombinant-antibodies.png)
Anti-AG-3 antibody [EPR19606] - BSA and Azide free (ab251346)