Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23120-25] to Ctip2 - BSA and Azide free
  • Suitable for: ICC/IF, IP, IHC-P, WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free
    See all Ctip2 primary antibodies

  • Description

    Rabbit monoclonal [EPR23120-25] to Ctip2 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Please note the species reactivity is application dependent.

    WB: Human, mouse and rat.

    ICC/IF: Human.

    IHC-P: Human, mouse and rat.

    FC: Human.

    IP: Human.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat whole cell lysate. Mouse and rat hippocampus and brain lysate. IHC-P: Human tonsil and spleen tissue. Mouse and rat spleen and hippocampus tissue. ICC/IF: Jurkat cells. Flow Cyt: Jurkat cells. IP: Jurkat whole cell lysate.

  • General notes

    ab269367 is the carrier-free version of ab240636. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Ctip2 with ab240636 at 1/500 dilution (0.96 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Nuclear staining on rat spleen (PMID: 30089823) is observed. The section was incubated with ab240636 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240636).

  • Immunoprecipitation - Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)
    Immunoprecipitation - Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)

    Ctip2 was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia T lymphocyte) whole cell lysate with ab240636 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab240636 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: Jurkat whole cell lysate 10µg

    Lane 2: ab240636 IP in Jurkat whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab240636 in Jurkat whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240636).

  • Immunocytochemistry/ Immunofluorescence - Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)
    Immunocytochemistry/ Immunofluorescence - Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Jurkat (human T cell leukemia T lymphocyte) and Daudi (human Burkitt's lymphoma lymphoblast) cells labeling Ctip2 with ab240636 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in Jurkat cells.

    Negative control: Daudi (PMID:23383087).

    ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240636).

  • Flow Cytometry - Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)
    Flow Cytometry - Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Daudi (human Burkitt's lymphoma lymphoblast, Left) / Jurkat (human T cell leukemia T lymphocyte, Right) cells labeling Ctip2 with ab240636 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: Daudi (PMID:23383087).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240636).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)

    Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling Ctip2 with ab240636 at 1/500 dilution (0.96 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Nuclear staining on rat hippocampus (PMID: 19955470, PMID: 25757017) is observed. The section was incubated with ab240636 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240636).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling Ctip2 with ab240636 at 1/500 dilution (0.96 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Nuclear staining on mouse hippocampus (PMID: 19955470, PMID: 25757017) is observed. The section was incubated with ab240636 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240636).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Ctip2 with ab240636 at 1/500 dilution (0.96 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Nuclear staining on mouse spleen (PMID: 30089823) is observed. The section was incubated with ab240636 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240636).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Ctip2 with ab240636 at 1/500 dilution (0.96 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Nuclear staining on human spleen (PMID: 30089823) is observed. The section was incubated with ab240636 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240636).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Ctip2 with ab240636 at 1/500 dilution (0.96 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Nuclear staining on human tonsil (PMID: 30089823) is observed. The section was incubated with ab240636 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240636).

  • Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)
    Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Ctip2 antibody [EPR23120-25] - BSA and Azide free (ab269367)

© 2021 Astana Biomed Group LLP. All rights reserved.