Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 110 563 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Ctip2 antibody [EPR23120-25] (ab240636)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23120-25] to Ctip2
Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-Ctip2 antibody [EPR23120-25]
See all Ctip2 primary antibodies
Description

Rabbit monoclonal [EPR23120-25] to Ctip2
Host species

Rabbit
Specificity

Please note the species reactivity is application dependent.

WB: Human, mouse and rat.

ICC/IF: Human.

IHC-P: Human, mouse and rat.

FC: Human.

IP: Human.

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Mouse Rat Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Jurkat whole cell lysate. Mouse and rat hippocampus and brain lysate. IHC-P: Human tonsil and spleen tissue. Mouse and rat spleen and hippocampus tissue. ICC/IF: Jurkat cells. Flow Cyt: Jurkat cells. IP: Jurkat whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23120-25
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] (ab240636)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Ctip2 with ab240636 at 1/500 dilution (0.96 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Nuclear staining on rat spleen (PMID: 30089823) is observed. The section was incubated with ab240636 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunoprecipitation - Anti-Ctip2 antibody [EPR23120-25] (ab240636)

Ctip2 was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia T lymphocyte) whole cell lysate with ab240636 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab240636 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: Jurkat whole cell lysate 10µg

Lane 2: ab240636 IP in Jurkat whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab240636 in Jurkat whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 1 second.
Immunocytochemistry/ Immunofluorescence - Anti-Ctip2 antibody [EPR23120-25] (ab240636)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Jurkat (human T cell leukemia T lymphocyte) and Daudi (human Burkitt's lymphoma lymphoblast) cells labeling Ctip2 with ab240636 at 1/100 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in Jurkat cells.

Negative control: Daudi (PMID:23383087).

ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Flow Cytometry - Anti-Ctip2 antibody [EPR23120-25] (ab240636)

Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Daudi (human Burkitt's lymphoma lymphoblast, Left) / Jurkat (human T cell leukemia T lymphocyte, Right) cells labeling Ctip2 with ab240636 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Negative control: Daudi (PMID:23383087).
Western blot - Anti-Ctip2 antibody [EPR23120-25] (ab240636)

All lanes : Anti-Ctip2 antibody [EPR23120-25] (ab240636) at 1/1000 dilution

Lane 1 : Mouse hippocampus tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Rat hippocampus tissue lysate
Lane 4 : Rat brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 96 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 23383087, 24407555).
Western blot - Anti-Ctip2 antibody [EPR23120-25] (ab240636)

All lanes : Anti-Ctip2 antibody [EPR23120-25] (ab240636) at 1/1000 dilution

Lane 1 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 2 : Daudi (human Burkitt's lymphoma lymphoblast), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 96 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 5.5 seconds.

Negative control: Daudi (PMID: 23383087).

Fresh lysates are recommended.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 23383087, 24407555).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] (ab240636)

Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling Ctip2 with ab240636 at 1/500 dilution (0.96 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Nuclear staining on rat hippocampus (PMID: 19955470, PMID: 25757017) is observed. The section was incubated with ab240636 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] (ab240636)

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling Ctip2 with ab240636 at 1/500 dilution (0.96 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Nuclear staining on mouse hippocampus (PMID: 19955470, PMID: 25757017) is observed. The section was incubated with ab240636 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] (ab240636)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Ctip2 with ab240636 at 1/500 dilution (0.96 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Nuclear staining on mouse spleen (PMID: 30089823) is observed. The section was incubated with ab240636 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] (ab240636)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Ctip2 with ab240636 at 1/500 dilution (0.96 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Nuclear staining on human spleen (PMID: 30089823) is observed. The section was incubated with ab240636 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [EPR23120-25] (ab240636)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Ctip2 with ab240636 at 1/500 dilution (0.96 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Nuclear staining on human tonsil (PMID: 30089823) is observed. The section was incubated with ab240636 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Anti-Ctip2 antibody [EPR23120-25] (ab240636)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com