Antibodies, Proteins, Kits and Reagents for Life Science

274 732 ₸ Product size

100 µg 274 732 ₸

Order now and get it on Thursday March 04, 2021

Key features and details

Mouse monoclonal [1F8G8] to Ctip2
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
Reacts with: Human
Isotype: IgG1

Product name

Anti-Ctip2 antibody [1F8G8]
See all Ctip2 primary antibodies
Description

Mouse monoclonal [1F8G8] to Ctip2
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human Ctip2 aa 1-150. (Expressed in E.coli).
Database link: Q9C0K0
Positive control
- WB: Recombinant human Ctip2 (aa1-150) protein; Ctip2 (aa1-150)-hIgG-Fc-transfected HEK-293 cell lysate. ICC/IF: HeLa cells. Flow cyt: HeLa and Jurkat cells. IHC-P: Human colon cancer and stomach cancer tissues.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.05% Sodium azide
Constituent: PBS
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from tissue culture supernatant.
Clonality

Monoclonal
Clone number

1F8G8
Isotype

IgG1
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [1F8G8] (ab233713)

Paraffin-embedded human colon cancer tissue stained for Ctip2 using ab233713 at 1/200 dilution in immunohistochemical analysis. DAB staining.
Western blot - Anti-Ctip2 antibody [1F8G8] (ab233713)

Anti-Ctip2 antibody [1F8G8] (ab233713) at 1/500 dilution + Recombinant human Ctip2 (aa1-150) protein

Predicted band size: 96 kDa
Western blot - Anti-Ctip2 antibody [1F8G8] (ab233713)

All lanes : Anti-Ctip2 antibody [1F8G8] (ab233713) at 1/500 dilution

Lane 1 : Non-transfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : Ctip2 (aa1-150)-hIgG-Fc-transfected HEK-293 cell lysate

Predicted band size: 96 kDa
Flow Cytometry - Anti-Ctip2 antibody [1F8G8] (ab233713)

Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Ctip2 with ab233713 at 1/200 dilution (green) compared with a negative control (red).
Flow Cytometry - Anti-Ctip2 antibody [1F8G8] (ab233713)

Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling Ctip2 with ab233713 at 1/200 dilution (green) compared with a negative control (red).
Immunocytochemistry/ Immunofluorescence - Anti-Ctip2 antibody [1F8G8] (ab233713)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Ctip2 (green) using ab233713 at 1/50 dilution in ICC/IF. Actin is stained with AlexaFluor^®555-Phalloidin (red). Blue: DRAQ5 DNA dye.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [1F8G8] (ab233713)

Paraffin-embedded human stomach cancer tissue stained for Ctip2 using ab233713 at 1/200 dilution in immunohistochemical analysis. DAB staining.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com