Anti-Ctip2 antibody (ab28448)
Key features and details
- Rabbit polyclonal to Ctip2
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Ctip2 antibody
See all Ctip2 primary antibodies -
Description
Rabbit polyclonal to Ctip2 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 850 - 950 of Human Ctip2.
Read Abcam's proprietary immunogen policy (Peptides available as ab32404)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab28448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IP Use at an assay dependent concentration. WB Use a concentration of 1 µg/ml. Detects a band of approximately 125,130 kDa (predicted molecular weight: 96 kDa). Target
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Function
Tumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. May be a key regulator of both differentiation and survival during thymocyte development. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element. -
Tissue specificity
Highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma. -
Sequence similarities
Contains 6 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 64919 Human
- Entrez Gene: 58208 Mouse
- Entrez Gene: 314423 Rat
- Omim: 606558 Human
- SwissProt: Q9C0K0 Human
- SwissProt: Q99PV8 Mouse
- Unigene: 709690 Human
- Unigene: 392694 Mouse
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Alternative names
- ATL1 alpha antibody
- ATL1 antibody
- ATL1 beta antibody
see all
Images
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Western blot - Anti-Ctip2 antibody (ab28448)All lanes : Anti-Ctip2 antibody (ab28448) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : Jurkat nuclear extract lysate (ab14844)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 96 kDa
Observed band size: 125,130 kDa why is the actual band size different from the predicted? -
Immunoprecipitation - Anti-Ctip2 antibody (ab28448)Ctip2 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to Ctip2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab28448.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Bands: 125, 130kDa: Ctip2.
Protocols
Datasheets and documents
References (29)
ab28448 has been referenced in 29 publications.
- Lee FHF et al. Altered cortical Cytoarchitecture in the Fmr1 knockout mouse. Mol Brain 12:56 (2019). PubMed: 31200759
- Bittermann E et al. Differential requirements of tubulin genes in mammalian forebrain development. PLoS Genet 15:e1008243 (2019). PubMed: 31386652
- Saxena M et al. Perturbation of canonical and non-canonical BMP signaling affects migration, polarity and dendritogenesis of mouse cortical neurons. Development 145:N/A (2018). PubMed: 29180570
- Maskari RA et al. Functional characterization of common BCL11B gene desert variants suggests a lymphocyte-mediated association of BCL11B with aortic stiffness. Eur J Hum Genet 26:1648-1657 (2018). PubMed: 30089823
- Kreutz A & Barger N Maximizing Explanatory Power in Stereological Data Collection: A Protocol for Reliably Integrating Optical Fractionator and Multiple Immunofluorescence Techniques. Front Neuroanat 12:73 (2018). PubMed: 30425623
Images
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Western blot - Anti-Ctip2 antibody (ab28448)All lanes : Anti-Ctip2 antibody (ab28448) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : Jurkat nuclear extract lysate (ab14844)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 96 kDa
Observed band size: 125,130 kDa why is the actual band size different from the predicted?
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Immunoprecipitation - Anti-Ctip2 antibody (ab28448)Ctip2 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to Ctip2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab28448.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Bands: 125, 130kDa: Ctip2.
