SIRT2 Screening Assay Kit (ab133082)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
Overview
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Product name
SIRT2 Screening Assay Kit
See all SIRT2 kits -
Detection method
Fluorescent -
Precision
Intra-assay Sample n Mean SD CV% Overall 16 3.8% Inter-assay Sample n Mean SD CV% Overall 16 3.9% -
Assay type
Quantitative -
Product overview
Abcam's SIRT2 Screening Assay Kit (ab133082) provides a convenient fluorescence-based method for screening SIRT2 inhibitors or activators. The procedure requires only two easy steps, both performed in the same microplate. In the first step, the substrate, which comprises the p53 sequence Gln-Pro-Lys-Lys(epsilon-acetyl)-AMC, is incubated with human recombinant SIRT2 along with its co-substrate NAD+. Deacetylation sensitizes the substrate such that treatment with the Developer in the second step releases a fluorescent product. The Fluorophore can be analyzed with an excitation wavelength of 350-360 nm and an emission wavelength of 450-465 nm.
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 96 tests 96-Well Plate Cover 1 unit Half Volume 96-Well Solid Plate (white) 1 unit SIRT2 (human recombinant) 2 vials SIRT2 Direct Assay Buffer (10X) 1 vial SIRT2 Direct Developer 1 vial SIRT2 Direct Fluorophore 1 vial SIRT2 Direct NAD+ 1 vial SIRT2 Direct Nicotinamide 1 vial SIRT2 Direct Peptide 2 vials -
Research areas
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Function
NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. -
Tissue specificity
Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. -
Sequence similarities
Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain. -
Developmental stage
Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome. -
Post-translational
modificationsPhosphorylated at the G2/M transition of the cell cycle. -
Cellular localization
Cytoplasm > cytoskeleton. Colocalizes with microtubules. - Information by UniProt
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Alternative names
- FLJ35621
- FLJ37491
- NAD dependent deacetylase sirtuin 2
see all
Images
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Functional Studies - SIRT2 Screening Assay Kit (ab133082)Inhibition of SIRT2 by nicotinamide (IC50 = 250 µM)
