SIRT2 Activity Assay Kit (Fluorometric) (ab156066)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
Overview
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Product name
SIRT2 Activity Assay Kit (Fluorometric)
See all SIRT2 kits -
Detection method
Fluorescent -
Assay type
Enzyme activity -
Species reactivity
Reacts with: Mouse, Rat, Human -
Product overview
Abcam’s SIRT2 Activity Assay Kit (Fluorometric) (ab156066) detects deacetylase activity of recombinant SIRT2.
Primarily, Abcam’s SIRT2 Activity Assay Kit (Fluorometric) is designed for the rapid and sensitive evaluation of SIRT2 inhibitors or activators using recombinant SIRT2 or purified SIRT2.
Applications for this kit include:
1. Screening inhibitors or activators of SIRT2.
2. Detecting the effects of pharmacological agents on SIRT2.
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Notes
Histone Deacetylases (HDACs) are a class of enzymes responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), allowing the histones to wrap the DNA more tightly.
HDAC proteins occur in four groups (class I, class IIA, class IIB, class III, class IV) based on function and DNA sequence similarity.
Classes I, IIA and IIB are considered "classical" HDACs whose activities are inhibited by trichostatin A (TSA), whereas class III is a family of NAD+-dependent proteins (sirtuins) not affected by TSA. Class IV is considered an atypical class on its own, based solely on DNA sequence similarity to the others. -
Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 100 tests Developer 1 x 500µl Fluoro-Deacetylated Peptide (0.2 mM) 1 x 100µl Fluoro-Substrate Peptide (0.2 mM) 1 x 500µl NAD (8 mM) 1 x 500µl Recombinant SIRT2 1 x 500µl SIRT Assay Buffer 2 x 1ml Stop Solution 2 x 1ml -
Research areas
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Function
NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. -
Tissue specificity
Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. -
Sequence similarities
Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain. -
Developmental stage
Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome. -
Post-translational
modificationsPhosphorylated at the G2/M transition of the cell cycle. -
Cellular localization
Cytoplasm > cytoskeleton. Colocalizes with microtubules. - Information by UniProt
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Alternative names
- FLJ35621
- FLJ37491
- NAD dependent deacetylase sirtuin 2
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Database links
- Entrez Gene: 22933 Human
- Entrez Gene: 64383 Mouse
- Entrez Gene: 361532 Rat
- Omim: 604480 Human
- SwissProt: Q8IXJ6 Human
- SwissProt: Q8VDQ8 Mouse
- SwissProt: Q5RJQ4 Rat
- Unigene: 466693 Human
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Images
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ab156066 - SIRT2 Activity Assay Kit (Fluorometric)Time course of SIRT2-substrate deacetylation by recombinant SIRT2
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ab156066 - SIRT2 Activity Assay Kit (Fluorometric)Effect of Sirtinol on recombinant SITR2 activity
