Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Wednesday March 17, 2021

Key features and details

Rabbit polyclonal to HDAC3
Suitable for: WB, IHC-P, ICC/IF, ChIP, IP
Reacts with: Mouse, Rat, Human
Isotype: IgG

Product name

Anti-HDAC3 antibody
See all HDAC3 primary antibodies
Description

Rabbit polyclonal to HDAC3
Host species

Rabbit

Tested Applications & Species

Application	Species
ChIP	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human HDAC3 aa 361-428 (C terminal).
Database link: O15379
Positive control
- WB: HEK-293T, A431, H1299, HeLaS3, HepG2, MOLT-4, and Raji whole cell lysate. Neuro2A , C8D30 , NIH/3T3 , Raw264.7 , C2C12 whole cell extracts. PC-12 , Rat2, U-87 MG , SK-N-SH , IMR32 , SK-N-AS whole cell exracts. ICC/IF: HeLa cells, SK-N-SH cells. IHC-P: SW480 xenograft. IP: HEK-293T cells.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Immunogen affinity purified
Clonality

Polyclonal
Isotype

IgG
Research areas

Western blot - Anti-HDAC3 antibody - C-terminal (ab137704)

All lanes : Anti-HDAC3 antibody (ab137704) at 1/1000 dilution

Lane 1 : HEK-293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lane 4 : HeLa S3 whole cell lysate
Lane 5 : HepG2 whole cell lysate
Lane 6 : MOLT-4 whole cell lysate
Lane 7 : Raji whole cell lysate

Lysates/proteins at 30 µg per lane.

Predicted band size: 48 kDa

10% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody - C-terminal (ab137704)

Immunohistochemistry analysis of paraffin-embedded SW480 xenograft labelling FGFR3 with ab137704 at 1/500.
Immunocytochemistry/ Immunofluorescence - Anti-HDAC3 antibody - C-terminal (ab137704)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labelling HDAC3 with ab137704 at 1/200.
ChIP - Anti-HDAC3 antibody (ab137704)

HDAC3 antibody immunoprecipitates HDAC3 protein-DNA in ChIP experiments. ChIP Sample: HEK-293T whole cell lysate/extract

A. 5 μg preimmune rabbit IgG

B. 5 μg of HDAC3 antibody. The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.
Immunoprecipitation - Anti-HDAC3 antibody (ab137704)

HDAC3 was immunoprecipitated from 1000µg of HEK-293T whole cell lysate with ab137704 at 1/1000 dilution. 10% SDS-PAGE. EasyBlot anti-rabbit IgG was used as secondary antibody.

Lane 1: 50 μg HEK-293T whole cell lysate/extract.
Lane 2: Control with 2 μg of preimmune rabbit IgG.
Lane 3: Immunoprecipitation of HDAC3 protein by 2 μg of HDAC3 antibody.
Western blot - Anti-HDAC3 antibody (ab137704)

All lanes : Anti-HDAC3 antibody (ab137704) at 1/500 dilution

Lane 1 : U-87 MG whole cell extract
Lane 2 : SK-N-SH whole cell extract
Lane 3 : IMR32 whole cell extract
Lane 4 : SK-N-AS whole cell extract

Lysates/proteins at 30 µg per lane.

Predicted band size: 48 kDa

10% SDS-PAGE
Immunocytochemistry/ Immunofluorescence - Anti-HDAC3 antibody (ab137704)

SK-N-SH cells stained for HDAC3 (green) using ab137704 at 1/400 dilution in ICC/IF.
Western blot - Anti-HDAC3 antibody (ab137704)

All lanes : Anti-HDAC3 antibody (ab137704) at 1/1000 dilution

Lane 1 : PC-12 whole cell extract
Lane 2 : Rat2 whole cell extract

Lysates/proteins at 30 µg per lane.

Predicted band size: 48 kDa

10% SDS-PAGE
Western blot - Anti-HDAC3 antibody (ab137704)

All lanes : Anti-HDAC3 antibody (ab137704) at 1/1000 dilution

Lane 1 : Neuro2A whole cell extract
Lane 2 : C8D30 whole cell extract
Lane 3 : NIH/3T3 whole cell extract
Lane 4 : Raw264.7 whole cell extract
Lane 5 : C2C12 whole cell extract

Lysates/proteins at 30 µg per lane.

Predicted band size: 48 kDa

10% SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com