Anti-NDUFS3 antibody (ab154543)
Key features and details
- Rabbit polyclonal to NDUFS3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NDUFS3 antibody
See all NDUFS3 primary antibodies -
Description
Rabbit polyclonal to NDUFS3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Monkey -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 60-264 of Human NDUFS3 (Uniprot ID: O75489).
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Positive control
- THP1 and HL-60 whole cell lysates. U87 xenograft.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-NDUFS3 antibody (ab154543) at 1/1000 dilution
Lane 1 : WT 293T cell extracts
Lane 2 : NDUFS3 Knockout 293T cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 30 kDa
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All lanes : Anti-NDUFS3 antibody (ab154543) at 1/1000 dilution
Lane 1 : THP1 whole cell lysate
Lane 2 : HL60 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 30 kDa
12% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded U87 xenograft tissue labeling NDUFS3 with ab154543 at 1/500 dilution.