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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class I Keratin

Anti-Keratin 77 antibody (ab106847)

Price and availability

264 681 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-Keratin 77 antibody (ab106847)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Keratin 77
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Keratin 77 antibody
    See all Keratin 77 primary antibodies
  • Description

    Rabbit polyclonal to Keratin 77
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human Keratin 77 aa 200-300 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab126633)

  • Positive control

    • This antibody gave a positive signal in both Human Skin and Placenta tissue lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class I
    • Keratin

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab106847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Gorilla
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 62 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 62 kDa).

Target

  • Tissue specificity

    Expressed exclusively in skin.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Post-translational
    modifications

    Undergoes deimination of some arginine residues (citrullination).
  • Target information above from: UniProt accession Q7Z794 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 374454 Human
    • Omim: 611158 Human
    • SwissProt: Q7Z794 Human
    • Unigene: 334989 Human
    • Alternative names

      • CK-1B antibody
      • Cytokeratin-1B antibody
      • K1B antibody
      • K2C1B_HUMAN antibody
      • K77 antibody
      • KB39 antibody
      • Keratin 1B antibody
      • Keratin, type II cytoskeletal 1b antibody
      • Keratin-77 antibody
      • KRT1B antibody
      • Krt77 antibody
      • Type-II keratin Kb39 antibody
      see all

    Images

    • Western blot - Anti-Keratin 77 antibody (ab106847)
      Western blot - Anti-Keratin 77 antibody (ab106847)
      All lanes : Anti-Keratin 77 antibody (ab106847) at 1 µg/ml

      Lane 1 : Human skin tissue lysate - total protein (ab30166)
      Lane 2 : Human placenta tissue lysate - total protein (ab29745)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 62 kDa


      Exposure time: 2 minutes

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab106847? Please let us know so that we can cite the reference in this datasheet.

    ab106847 has not yet been referenced specifically in any publications.

    Images

    • Western blot - Anti-Keratin 77 antibody (ab106847)
      Western blot - Anti-Keratin 77 antibody (ab106847)
      All lanes : Anti-Keratin 77 antibody (ab106847) at 1 µg/ml

      Lane 1 : Human skin tissue lysate - total protein (ab30166)
      Lane 2 : Human placenta tissue lysate - total protein (ab29745)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 62 kDa


      Exposure time: 2 minutes

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Alternative products to Anti-Keratin 77 antibody (ab106847)

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    • Product image

      Anti-Keratin 77 antibody (ab126329)

      Applications: IHC-P

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