All lanes : Anti-NDUFS3 antibody [3F9DD2] (ab110246) at 1/1000 dilution

Lanes 1-2 : Whole cell lysates from NDUFS3 KO cells.
Lanes 3-4 : Whole cell lysates from NDUFS3 WT cells.
Lanes 5-6 : Isolated mitochondria from NDUFS3 KO cells.
Lanes 7-8 : Isolated mitochondria from NDUFS3 WT cells.

Lysates/proteins at 50 µg per lane.

Secondary
All lanes : Goat anti-Mouse monoclonal HRP conjugate at 1/5000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 30 kDa
Additional bands at: 260 kDa (possible non-specific binding), 40 kDa (possible non-specific binding)


Exposure time: 1 minute

See Abreview

All lanes : Anti-NDUFS3 antibody [3F9DD2] (ab110246) at 0.5 µg/ml

Lane 1 : Isolated mitochondria from Human heart at 5 µg
Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
Lane 3 : Isolated mitochondria from Rat heart at 10 µg
Lane 4 : Isolated mitochondria from Mouse heart at 10 µg

Predicted band size: 30 kDa

ab110246, at 10 µg/ml, staining NDUFS3 in cultured Human fibroblasts by Immunofluorescence. The cells were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110246, followed by a fluorescent goat-anti-mouse IgG.

ab110246, at 1 µg/ml, staining NDUFS3 in HeLa cells by Flow Cytometry (Blue).

Isotype control antibody, at 1 µg/ml, staining (red).

ab110246, at 1/100, staining NDUFS3 in Human colon tissue fixed with 4% PFA by Immunohistochemistry.

ab110246 was labeled with a universal probe (which recognizes antibodies raised in both mouse and rabbit species) for 30 minutes and a HRP-polymer probe for 30 minutes. ab110246 was diluted and incubated for 1 hour with the target. Antigen retrieval was not required.

ab110246, at 1/1000, staining NDUFS3 in formalin fixed and paraffin embedded Human cerebellum tissue by Immunohistochemistry.

ab110246 was labeled with a universal probe (which recognizes antibodies raised in both mouse and rabbit species) for 30 minutes and a HRP-polymer probe for 30 minutes. ab110246 was diluted and incubated with the target for 1 hour. Antigen retrieval performed by 1 min pressure cooking with 1mmol EDTA, pH8.

ab110246 staining NDUFS3 in Human colon tissue sections from a normal ageing patient by Immunohistochemistry. Kindly provided by Dr. L. Greaves and D. Turnbull, Mitochondrial Research Group, Newcastle University. For more details, see Taylor et al., J. Clin. Invest. 112:1351-1360 (2003).