Anti-D2HGDH antibody (ab233516)
Key features and details
- Rabbit polyclonal to D2HGDH
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-D2HGDH antibody
See all D2HGDH primary antibodies -
Description
Rabbit polyclonal to D2HGDH -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human D2HGDH aa 50-250. Expressed in E. coli. N-terminal tags.
Database link: Q8N465 -
Positive control
- WB: Recombinant human D2HGDH protein; HEK-293T whole cell lysate; Rat kidney and liver tissue lysate. IHC-P: Human prostate cancer, breast cancer, cerebrum, colorectal cancer, glioma and liver tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-D2HGDH antibody (ab233516)Anti-D2HGDH antibody (ab233516) at 1 µg/ml + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Secondary
HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/ml
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D2HGDH antibody (ab233516)Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for D2HGDH using ab233516 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D2HGDH antibody (ab233516)Formalin-fixed, paraffin-embedded human breast cancer tissue stained for D2HGDH using ab233516 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D2HGDH antibody (ab233516)Formalin-fixed, paraffin-embedded human cerebrum tissue stained for D2HGDH using ab233516 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D2HGDH antibody (ab233516)Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for D2HGDH using ab233516 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D2HGDH antibody (ab233516)Formalin-fixed, paraffin-embedded human glioma tissue stained for D2HGDH using ab233516 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D2HGDH antibody (ab233516)Formalin-fixed, paraffin-embedded human liver tissue stained for D2HGDH using ab233516 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D2HGDH antibody (ab233516)Formalin-fixed, paraffin-embedded human testis tissue stained for D2HGDH using ab233516 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Western blot - Anti-D2HGDH antibody (ab233516)Anti-D2HGDH antibody (ab233516) at 1 µg/ml + Rat kidney tissue lysate
Secondary
HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/ml
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Western blot - Anti-D2HGDH antibody (ab233516)Anti-D2HGDH antibody (ab233516) at 1 µg/ml + Rat liver tissue lysate
Secondary
HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/ml
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Western blot - Anti-D2HGDH antibody (ab233516)Anti-D2HGDH antibody (ab233516) at 2 µg/ml + Recombinant human D2HGDH protein