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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Ras Family

Human RHOC Matched Antibody Pair Kit (ab221426)

Human RHOC Matched Antibody Pair Kit (ab221426)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
  • For economical ELISA and ELISA-based assay development
  • Reacts with: Human
  • Range: 31.2 pg/ml - 2000 pg/ml

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Overview

  • Product name

    Human RHOC Matched Antibody Pair Kit
    See all RHOC kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Sensitivity

    6.5 pg/ml
  • Range

    31.2 pg/ml - 2000 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    Huam RHOC Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human RHOC.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


    Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab218263. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 5 x 96 tests
    Human RHOC Capture Antibody 2 x 50µg 1 x 50µg
    Human RHOC Detector Antibody 2 x 12.5µg 1 x 12.5µg
    Human RHOC Lyophilized Protein 2 vials 1 vial
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Signal transducers
  • Function

    Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Rho family.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P08134 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Aplysia RAS-related homolog 9
    • ARH 9
    • ARH9
    • ARHC
    • H9
    • MGC1448
    • MGC61427
    • Oncogene RHO H9
    • Ras homolog gene family member C
    • RAS related homolog 9
    • RHO C
    • Rho cDNA clone 9
    • Rho related GTP binding protein RhoC
    • Rho-related GTP-binding protein RhoC
    • rhoC
    • RhoC GTPase
    • RHOC_HUMAN
    • RHOH 9
    • RHOH9
    • Small GTP binding protein RhoC
    see all
  • Database links

    • Entrez Gene: 389 Human
    • Omim: 165380 Human
    • SwissProt: P08134 Human
    • Unigene: 502659 Human
    • Unigene: 658289 Human

    Images

    • Human RHOC standard curve
      Human RHOC standard curve

      Standard calibration curve. Background subtracted values are graphed.

    • Sandwich ELISA - Human RHOC Matched Antibody Pair Kit (ab221426)
      Sandwich ELISA - Human RHOC Matched Antibody Pair Kit (ab221426)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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