Antibodies, Proteins, Kits and Reagents for Life Science

154 118 ₸ Product size

100 µl 154 118 ₸

Order now and get it on Tuesday March 02, 2021

Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [MJF-R21] to RAB10 (phospho T73)
Suitable for: WB, Dot blot
Knockout validated
Reacts with: Mouse, Human, Recombinant fragment

Product name

Anti-RAB10 (phospho T73) antibody [MJF-R21]
See all RAB10 primary antibodies
Description

Rabbit monoclonal [MJF-R21] to RAB10 (phospho T73)
Host species

Rabbit

Tested Applications & Species

Application	Species
Dot	Recombinant fragment
WB	Mouse Human Recombinant fragment

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Wild-type MEF whole cell lysate; LRRK2 [R1441C] knock-in MEF whole cell lysate. Dot Blot: Rab10 (phospho T73) peptide.
General notes
Please see PMID: 29127256. Lis P et al. Development of phospho-specific Rab protein antibodies to monitor in vivo activity of the LRRK2 Parkinson's disease kinase. Biochem J 475:1-22 (2018).

This antibody was developed with support from The Michael J. Fox Foundation.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

MJF-R21
Isotype

IgG
Research areas

Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261)

All lanes : Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261) at 1/1000 dilution

Lane 1 : Wild-type MEF (mouse embryonic fibroblast cell line) whole cell lysate
Lane 2 : Wild-type MEF treated with 100 nM MLi-2 for 90 minutes, whole cell lysate
Lane 3 : LRRK2 [R1441C] knock-in MEF whole cell lysate
Lane 4 : LRRK2 [R1441C] knock-in MEF treated with 100 nM MLi-2 for 90 minutes, whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 23 kDa
Observed band size: 23 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

The LRRK2 pathogenic mutation R1441C increases LRRK2 activity and markedly elevates Rab10 phosphorylation in MEF (mouse embryonic fibroblasts).

The expression pattern is consistent with the literature (PMID: 29127256).

The cell lysates were kindly provided by our collaborator, Dr. Dario Alessi.
Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261)

All lanes : Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261) at 1/1000 dilution

Lane 1 : Wild-type A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : Wild-type A549 treated with 100 nM MLi-2 for 90 minutes, whole cell lysate
Lane 3 : Rab8A knock-out A549 whole cell lysate
Lane 4 : Rab8A knock-out A549 treated with 100 nM MLi-2 for 90 minutes, whole cell lysate
Lane 5 : Rab10 knock-out A549 whole cell lysate
Lane 6 : Rab10 knock-out A549 treated with 100 nM MLi-2 for 90 minutes, whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP-labeled secondary antibody at 1/2500 dilution

Predicted band size: 23 kDa
Observed band size: 23 kDa

Blocking buffer: 5% NFDM/TBST.

Dilution buffer: 5% BSA/TBST.

The images were kindly provided by our collaborator, Dr. Dario Alessi, and have been published (PMID: 29127256).

Scanned with Licor Odyssey CLx.
Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261)

All lanes : Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261) at 1/1000 dilution

Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cells transfected with LRRK2 [Y1699C] and HA-tagged Rab3A expression vectors, were treated with 150 nM MLi-2 for 90 minutes, whole cell lysate
Lane 2 : HEK-293 cells transfected with LRRK2 [Y1699C] and HA-tagged Rab8A expression vectors, were treated with 150 nM MLi-2 for 90 minutes, whole cell lysate
Lane 3 : HEK-293 cells transfected with LRRK2 [Y1699C] and HA-tagged Rab10 expression vectors, were treated with 150 nM MLi-2 for 90 minutes, whole cell lysate
Lane 4 : HEK-293 cells transfected with LRRK2 [Y1699C] and HA-tagged Rab35 expression vectors, were treated with 150 nM MLi-2 for 90 minutes, whole cell lysate
Lane 5 : HEK-293 cells transfected with LRRK2[Y1699C] and HA-tagged Rab43 expression vectors, were treated with 150 nM MLi-2 for 90 minutes, whole cell lysate

Lysates/proteins at 0.1 µg per lane.

Secondary
All lanes : IRDye 800CW secondary antibody at 1/25000 dilution

Predicted band size: 23 kDa
Observed band size: 23 kDa

Blocking buffer: 5% NFDM/TBST.

Dilution buffer: 5% BSA/TBST.

The LRRK2 pathogenic mutation Y1699C increases LRRK2 activity and markedly elevates the phosphorylation of Rab proteins.

The images were kindly provided by our collaborator Dr. Dario Alessi, and have been published (PMID: 29127256).

Scanned with Licor Odyssey CLx.
Dot Blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261)

Dot blot analysis of Rab10 (phospho T73) labeled with ab230261 at 1/1000 dilution.

Lane 1: Rab10 (phospho T73) peptide;

Lane 2: Rab10 non-phospho peptide.

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100,000 dilution was used as secondary antibody.

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: 32 seconds.
Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261)

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