Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Tuesday March 02, 2021

Anti-RAB10 antibody [EPR13242] (ab181367)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13242] to RAB10
Suitable for: WB, Flow Cyt
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-RAB10 antibody [EPR13242]
See all RAB10 primary antibodies
Description

Rabbit monoclonal [EPR13242] to RAB10
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Mouse

Human

See all applications and species data
Immunogen

Recombinant fragment within Human RAB10 aa 50-200. The exact sequence is proprietary.
Database link: P61026
Positive control
- WB: NIH/3T3, C6, U-87 MG, MCF7, A549 and HeLa whole cell lysates Flow cyt: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
Flow Cyt	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR13242
Isotype

IgG
Research areas

Western blot - Anti-RAB10 antibody [EPR13242] (ab181367)

All lanes : Anti-RAB10 antibody [EPR13242] (ab181367) at 1/1000 dilution

Lane 1 : Wild-type A549 whole cell lysate
Lane 2 : RAB10 knockout A549 whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : MCF7 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 22 kDa
Observed band size: 25 kDa
why is the actual band size different from the predicted?

Lanes 1 - 4: Merged signal (red and green). Green - ab181367 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab181367 was shown to specifically react with RAB10 in wild-type A549 cells as signal was lost in RAB10 knockout cells. Wild-type and RAB10 knockout samples were subjected to SDS-PAGE. Ab181367 and ab8245(Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-RAB10 antibody [EPR13242] (ab181367)

All lanes : Anti-RAB10 antibody [EPR13242] (ab181367) at 1/1000 dilution (Purified)

Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 22 kDa
Observed band size: 22 kDa
Flow Cytometry - Anti-RAB10 antibody [EPR13242] (ab181367)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RAB10 with Purified ab181367 at 1:20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-RAB10 antibody [EPR13242] (ab181367)

All lanes : Anti-RAB10 antibody [EPR13242] (ab181367) at 1/2000 dilution (Purified)

Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 2 : C6 (Rat glial tumor glial cell) whole cell lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 22 kDa
Observed band size: 22 kDa
Anti-RAB10 antibody [EPR13242] (ab181367)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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