Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RAB10 (phospho T73) antibody [MJF-R21] - BSA and Azide free (ab231707)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [MJF-R21] to RAB10 (phospho T73) - BSA and Azide free
  • Suitable for: WB, Dot blot
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-RAB10 (phospho T73) antibody [MJF-R21] - BSA and Azide free
    See all RAB10 primary antibodies

  • Description

    Rabbit monoclonal [MJF-R21] to RAB10 (phospho T73) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Dot
    Recombinant fragment
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild-type MEF whole cell lysate; LRRK2 [R1441C] knock-in MEF whole cell lysate.

  • General notes

    ab231707 is the carrier-free version of ab230261 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab231707 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Please see PMID: 29127256. Lis P et al. Development of phospho-specific Rab protein antibodies to monitor in vivo activity of the LRRK2 Parkinson's disease kinase. Biochem J 475 :1-22 (2018).

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Dot Blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] - BSA and Azide free (ab231707)
    Dot Blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] - BSA and Azide free (ab231707)

    Dot blot analysis of Rab10 (phospho T73) labeled with ab230261 at 1/1000 dilution.

    Lane 1: Rab10 (phospho T73) peptide;

    Lane 2: Rab10 non-phospho peptide.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100,000 dilution was used as secondary antibody.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 32 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230261).

  • Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] - BSA and Azide free (ab231707)
    Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] - BSA and Azide free (ab231707)
    All lanes : Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261) at 1/1000 dilution

    Lane 1 : Wild-type MEF (mouse embryonic fibroblast cell line) whole cell lysate
    Lane 2 : Wild-type MEF treated with 100 nM MLi-2 for 90 minutes, whole cell lysate
    Lane 3 : LRRK2 [R1441C] knock-in MEF whole cell lysate
    Lane 4 : LRRK2 [R1441C] knock-in MEF treated with 100 nM MLi-2 for 90 minutes, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    The LRRK2 pathogenic mutation R1441C increases LRRK2 activity and markedly elevates RAB10 phosphorylation in MEF (mouse embryonic fibroblasts).

    The expression pattern is consistent with the literature (PMID: 29127256).

    The cell lysates were kindly provided by our collaborator, Dr. Dario Alessi.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230261).

  • Anti-RAB10 (phospho T73) antibody [MJF-R21] - BSA and Azide free (ab231707)
    Anti-RAB10 (phospho T73) antibody [MJF-R21] - BSA and Azide free (ab231707)

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