Antibodies, Proteins, Kits and Reagents for Life Science

308 236 ₸ Product size

100 µl 308 236 ₸

Order now and get it on Wednesday March 10, 2021

Key features and details

Mouse monoclonal [4E2] to RAB10
Suitable for: ICC/IF, WB, Flow Cyt
Reacts with: Mouse, Human
Isotype: IgG1

Product name

Anti-RAB10 antibody [4E2]
See all RAB10 primary antibodies
Description

Mouse monoclonal [4E2] to RAB10
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Purified recombinant fragment of Human RAB10 expressed in E. coli.
Positive control
- Hela and NIH/3T3 cell lysates; LOVO cells
General notes

This product was changed from ascites to supernatant. Lot no’s high than GR120151-16 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
Storage buffer

Preservative: 0.05% Sodium azide
Constituent: PBS
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from tissue culture supernatant.
Clonality

Monoclonal
Clone number

4E2
Isotype

IgG1
Research areas

Western blot - Anti-RAB10 antibody [4E2] (ab104859)

All lanes : Anti-RAB10 antibody [4E2] (ab104859) at 1/500 dilution

Lane 1 : Hela cell lysate
Lane 2 : NIH/3T3 cell lysate

Predicted band size: 23 kDa
Immunocytochemistry/ Immunofluorescence - Anti-RAB10 antibody [4E2] (ab104859)

Immunofluorescence analysis of LOVO cells using ab104859 (green). Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry - Anti-RAB10 antibody [4E2] (ab104859)

Overlay histogram showing HeLa cells stained with ab104859 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104859, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91653, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com