Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RAB10 antibody [4E2] (ab104859)

Key features and details

  • Mouse monoclonal [4E2] to RAB10
  • Suitable for: ICC/IF, WB, Flow Cyt
  • Reacts with: Mouse, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-RAB10 antibody [4E2]
    See all RAB10 primary antibodies

  • Description

    Mouse monoclonal [4E2] to RAB10

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Purified recombinant fragment of Human RAB10 expressed in E. coli.

  • Positive control

    • Hela and NIH/3T3 cell lysates; LOVO cells

  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR120151-16 are from Tissue Culture Supernatant

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-RAB10 antibody [4E2] (ab104859)
    Western blot - Anti-RAB10 antibody [4E2] (ab104859)
    All lanes : Anti-RAB10 antibody [4E2] (ab104859) at 1/500 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : NIH/3T3 cell lysate

    Predicted band size: 23 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RAB10 antibody [4E2] (ab104859)
    Immunocytochemistry/ Immunofluorescence - Anti-RAB10 antibody [4E2] (ab104859)
    Immunofluorescence analysis of LOVO cells using ab104859 (green). Blue: DRAQ5 fluorescent DNA dye.
  • Flow Cytometry - Anti-RAB10 antibody [4E2] (ab104859)
    Flow Cytometry - Anti-RAB10 antibody [4E2] (ab104859)
    Overlay histogram showing HeLa cells stained with ab104859 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104859, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91653, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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