Anti-Peripherin antibody [8G2] (ab4653)
![Anti-Peripherin antibody [8G2] (ab4653)](https://www.abcam.com/ps/products/4/ab4653/Images/ab4653-308038-anti-peripherin-antibody-8g2-western-blot.jpg "Anti-Peripherin antibody [8G2] (ab4653)")
Key features and details
- Mouse monoclonal [8G2] to Peripherin
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG1
Overview
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Product name
Anti-Peripherin antibody [8G2]
See all Peripherin primary antibodies -
Description
Mouse monoclonal [8G2] to Peripherin -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human -
Immunogen
Recombinant Rat Peripherin purified from E. coli.
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Positive control
- WB: Mouse, rat, cow spinal cord lysate, SH-SY5Y, PC-12 whole cell lysate. ICC: SH-SY5Y, PC-12 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.032% Sodium azide
Constituents: 49.98% Glycerol, 49.98% PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified antibody from TCS. -
Clonality
Monoclonal -
Clone number
8G2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-Peripherin antibody [8G2] (ab4653)All lanes : Anti-Peripherin antibody [8G2] (ab4653) at 1/500 dilution
Lane 2 : Mouse spinal cord lysate
Lane 3 : Rat spinal cord lysate
Lane 4 : Cow spinal cord lysate
Lane 5 : SH-SY5Y (human bone marrow neuroblastoma cell) whole cell lysate
Lane 6 : PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate
Secondary
All lanes : Anti-Mouse IgG
Predicted band size: 57 kDa
Observed band size: 57 kDa
Additional bands at: 25 kDa (possible IgG), 50 kDa (possible IgG)The band at ~57kDa corresponds to the peripherin protein. Bands at 50 and 25kDa detected in the mouse spinal cord lysate, correspond to the heavy and light chains of mouse IgG.
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![Immunocytochemistry/ Immunofluorescence - Anti-Peripherin antibody [8G2] (ab4653)](https://www.abcam.com/ps/products/4/ab4653/Images/ab4653-308037-anti-peripherin-antibody-8g2-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Peripherin antibody [8G2] (ab4653)Immunofluorescent analysis of SH-SY5Y (human bone marrow neuroblastoma cell) cells (A) and PC-12 (rat adrenal gland pheochromocytoma cell) cells (B), stained with ab4653 at 1/500 dilution (Red) and costained with chicken polyclonal anti-vimentin antibody at 1/10,000 dilution (Green). Blue: DAPI staining of nuclear DNA.
