Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Laminin 2 alpha antibody [EPR21890] - BSA and Azide free (ab238458)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21890] to Laminin 2 alpha - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Laminin 2 alpha antibody [EPR21890] - BSA and Azide free
    See all Laminin 2 alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR21890] to Laminin 2 alpha - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human cardiac muscle tissue.

  • General notes

    Ab238458 is the carrier-free version of ab219409. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238458 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 2 alpha antibody [EPR21890] - BSA and Azide free (ab238458)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 2 alpha antibody [EPR21890] - BSA and Azide free (ab238458)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Laminin 2 alpha with ab219409 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on villous basal membrane of human placenta is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219409).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 2 alpha antibody [EPR21890] - BSA and Azide free (ab238458)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 2 alpha antibody [EPR21890] - BSA and Azide free (ab238458)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Laminin 2 alpha with ab219409 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on basal lamina of human skeletal muscle (PMID: 26355035). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219409).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 2 alpha antibody [EPR21890] - BSA and Azide free (ab238458)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 2 alpha antibody [EPR21890] - BSA and Azide free (ab238458)

    Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Laminin 2 alpha with ab219409 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on basal lamina of human cardiac muscle (PMID: 10773239). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab219409).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

     

  • Anti-Laminin 2 alpha antibody [EPR21890] - BSA and Azide free (ab238458)
    Anti-Laminin 2 alpha antibody [EPR21890] - BSA and Azide free (ab238458)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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