Anti-Laminin 2 alpha antibody [4H8-2] (ab11576)
Key features and details
- Rat monoclonal [4H8-2] to Laminin 2 alpha
- Suitable for: IHC-Fr
- Reacts with: Mouse
- Isotype: IgG1
Overview
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Product name
Anti-Laminin 2 alpha antibody [4H8-2]
See all Laminin 2 alpha primary antibodies -
Description
Rat monoclonal [4H8-2] to Laminin 2 alpha -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species IHC-Fr Mouse -
Immunogen
Heart Laminin 2 (Mouse).
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Epitope
The epitope recognized by the antibody resides in the N-terminal portion of the alpha 2 chain of laminin. -
Positive control
- IHC-Fr: Mouse tongue tissue.
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General notes
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
4H8-2 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemical analysis of acetone-fixed frozen section of mouse tongue tissue labeling Laminin 2 alpha using ab11576 at 8 μg/mL, followed by a monoclonal Anti-Rat Kappa & Lamda light chains-FITC congugate at 1/40 dilution (Green).
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ab11576 staining C57B6 mouse skeletal muscle cryostate tissue sections by IHC-Fr. The section was acetone fixed and blocked with 5% BSA for 30 mintes at RT, prior to incubation with the primary antibody (diluted 1/200 in blocking buffer) for 2 hours. A rhodamine-conjugated goat anti-rat antibody was used as the secondary.