ab33922 showing positive staining in Normal brain tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry analysis of Embryonic mouse primary neural cells labeling GFAP with purified ab33922 at 1:100 dilution (10 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor^© 488, ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. Cells were counterstained with Tau Mouse mAb 1:100 (5 μg/ml). ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) was used as the secondary antibody for the counter stain at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. 

Confocal image showing positive staining in glial cells.

ICC image of ab33922 stained Rat primary mixed astrocytes culture. The cells were 100% Paraformaldehyde fixed and then incubated in 10% Serum / 0.1M PBS with 10% Donkey serum for 4h. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution.

ab33922 showing positive staining in Astrocytoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ab33922 showing negative staining in Meningioma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.