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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments

Anti-GFAP antibody [5C10] (ab190288)

Price and availability

291 484 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-GFAP antibody [5C10] (ab190288)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [5C10] to GFAP
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Rat, Horse, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-GFAP antibody [5C10]
    See all GFAP primary antibodies
  • Description

    Mouse monoclonal [5C10] to GFAP
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Horse
    WB
    Rat
    See all applications and species data
  • Immunogen

    Full length native protein (purified) corresponding to Pig GFAP. Purified form Pig spinal cord.

  • Positive control

    • Rat spinal cord protein extract; Mixed neuron-glial culture; Adult horse brain.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: 100% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    5C10
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Intermediate Filaments
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Astrocyte marker
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class II
    • GFAP
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Glial
    • Stem Cells
    • Neural Stem Cells
    • Glial Restricted Lineage
    • Astrocyte

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [5C10] (ab190288)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [5C10] (ab190288)

    Immunohistochemical analysis of formalin-fixed adult horse brain labeling GFAP with ab190288 at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [5C10] (ab190288)
    Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [5C10] (ab190288)

    Immunofluorescent analysis of mixed neuron-glial cultures labeling GFAP with ab190288 at 1/1000 dilution. Neurofilaments are stained in green; the blue channel shows the localization of DNA. 

  • Western blot - Anti-GFAP antibody [5C10] (ab190288)
    Western blot - Anti-GFAP antibody [5C10] (ab190288)
    Anti-GFAP antibody [5C10] (ab190288) at 1/5000 dilution + Rat spinal cord protein extract

    Predicted band size: 49 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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