Anti-Ezrin antibody [6F1A9] (ab205381)
Key features and details
- Mouse monoclonal [6F1A9] to Ezrin
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-Ezrin antibody [6F1A9]
See all Ezrin primary antibodies -
Description
Mouse monoclonal [6F1A9] to Ezrin -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Human -
Immunogen
Recombinant fragment corresponding to Human Ezrin aa 250-500. Expressed in E. Coli.
Database link: P15311 -
Positive control
- Human Ezrin (AA: 292-464) recombinant protein; Ezrin (AA: 292-464)-hIgGFc transfected HEK293 cell lysate; MCF7, HeLa, A431, HEK293, SK N SH, Jurkat, HepG2, NIH 3T3 and COS7 cell lysates; A549 cells; MCF7 cells and HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
6F1A9 -
Isotype
IgG1 -
Research areas
Images
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Anti-Ezrin antibody [6F1A9] (ab205381) at 1/500 dilution + Human Ezrin (AA: 292-464) recombinant protein.
Predicted band size: 69 kDaExpected MW is 47.1 kDa
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All lanes : Anti-Ezrin antibody [6F1A9] (ab205381) at 1/500 dilution
Lane 1 : Non-transfected HEK293 cell lysate.
Lane 2 : Ezrin (AA: 292-464)-hIgGFc transfected HEK293 cell lysate.
Predicted band size: 69 kDa
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All lanes : Anti-Ezrin antibody [6F1A9] (ab205381) at 1/500 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HEK293 cell lysate
Lane 5 : SK N SH cell lysate
Lane 6 : Jurkat cell lysate
Lane 7 : HepG2 cell lysate
Lane 8 : NIH 3T3 cell lysate
Lane 9 : COS-7 cell lysate
Predicted band size: 69 kDa
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Immunofluorescent analysis of A549 cells labeling Ezrin using ab205381 at 1/100 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
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Flow cytometric analysis of MCF7 cells labeling Ezrin using ab205381 at 1/200 dilution (green) and negative control (red).
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Flow cytometric analysis of HeLa cells labeling Ezrin using ab205381 at 1/200 dilution (green) and negative control (red).