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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments

Alexa Fluor® 647 Anti-Nestin antibody [rat-401] (ab196693)

Price and availability

314 937 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Alexa Fluor® 647 Anti-Nestin antibody [rat-401] (ab196693)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Alexa Fluor® 647 Mouse monoclonal [rat-401] to Nestin
  • Suitable for: ICC/IF
  • Reacts with: Rat
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Isotype: IgG1

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Overview

  • Product name

    Alexa Fluor® 647 Anti-Nestin antibody [rat-401]
    See all Nestin primary antibodies
  • Description

    Alexa Fluor® 647 Mouse monoclonal [rat-401] to Nestin
  • Host species

    Mouse
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested Applications & Species

    Application Species
    ICC/IF
    Rat
    See all applications and species data
  • Immunogen

    Tissue, cells or virus corresponding to Rat Nestin. Homogenized spinal cord tissue from embryonic day 15 (E15) rats.

  • Positive control

    • ICC/IF: PC12 cells
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Monoclonal
  • Clone number

    rat-401
  • Myeloma

    NS1
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Intermediate Filaments
    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Cardiovascular
    • Angiogenesis
    • Angiogenic Factors
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Stem Cells
    • Neural Stem Cells
    • Intracellular
    • Cancer
    • Tumor biomarkers
    • Tumor antigens
    • Stem Cells
    • Neural Stem Cells
    • Neuron Restricted Lineage
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Other
    • Developmental Biology
    • Lineage specification
    • Ectoderm

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Nestin antibody [rat-401] (ab196693)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Nestin antibody [rat-401] (ab196693)

    ab196693 staining Nestin in PC12 cells. The cells were fixed with 100% methanol (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab196693 at 1/50 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/167 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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