Antibodies, Proteins, Kits and Reagents for Life Science

274 732 ₸ Product size

100 µg 274 732 ₸

Order now and get it on Wednesday March 03, 2021

Key features and details

Product name

Anti-Nestin antibody
See all Nestin primary antibodies
Description

Rabbit polyclonal to Nestin
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Rat

See all applications and species data
Immunogen

Synthetic peptide corresponding to Rat Nestin aa 1550-1650 conjugated to keyhole limpet haemocyanin.
(Peptide available as ab103766)

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS

Batches of this product that have a concentration
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Purity

Immunogen affinity purified
Clonality

Polyclonal
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab93157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application	Species
IHC-P	Rat

Application	Abreviews	Notes
IHC-P	(1)	Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Function

Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Tissue specificity

CNS stem cells.
Sequence similarities

Belongs to the intermediate filament family.
Developmental stage

Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
Post-translational
modifications

Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
Target information above from: UniProt accession P48681 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- SwissProt: P21263 Rat
- Unigene: 9701 Rat
Alternative names
- ESTM 46 antibody
- FLJ 21841 antibody
- FLJ21841 antibody
- Intermediate filament protein antibody
- Nbla00170 antibody
- nes antibody
- NEST_HUMAN antibody
- Nestin antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody (ab93157)

IHC image of Nestin staining in Rat brain (6 weeks) FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93157, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Publishing research using ab93157? Please let us know so that we can cite the reference in this datasheet.

ab93157 has been referenced in 5 publications.

Oudega M et al. Validation study of neurotrophin-3-releasing chitosan facilitation of neural tissue generation in the severely injured adult rat spinal cord. Exp Neurol 312:51-62 (2019). PubMed: 30471251
Salehi MS et al. Dimethyl fumarate up-regulates expression of major neurotrophic factors in the epidermal neural crest stem cells. Tissue Cell 56:114-120 (2019). PubMed: 30736899
Pandamooz S et al. Enhancing the expression of neurotrophic factors in epidermal neural crest stem cells by valproic acid: A potential candidate for combinatorial treatment. Neurosci Lett 704:8-14 (2019). PubMed: 30904572
Fernández-Susavila H et al. Generation and characterization of the human iPSC line IDISi001-A isolated from blood cells of a CADASIL patient carrying a NOTCH3 mutation. Stem Cell Res 28:16-20 (2018). ICC/IF . PubMed: 29414412
Jia J et al. Recombinant PEP-1-SOD1 improves functional recovery after neural stem cell transplantation in rats with traumatic brain injury. Exp Ther Med 15:2929-2935 (2018). PubMed: 29599832

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody (ab93157)

IHC image of Nestin staining in Rat brain (6 weeks) FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93157, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.