Anti-PUF60/FIR antibody (ab22819)
Key features and details
- Goat polyclonal to PUF60/FIR
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-PUF60/FIR antibody
See all PUF60/FIR primary antibodies -
Description
Goat polyclonal to PUF60/FIR -
Host species
Goat -
Specificity
ab22819 recognises both reported human isoforms of PUF60/FIR
-
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Pig
-
Immunogen
Synthetic peptide corresponding to Human PUF60/FIR aa 530-542 (C terminal).
Sequence:YDQERFDNSDLSA
-
Positive control
-
General notes
This product was previously labelled as PUF60
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab22819 has bee purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-PUF60/FIR antibody (ab22819)Anti-PUF60/FIR antibody (ab22819) at 0.1 µg/ml + HEK293 cell lysate (35µg in RIPA buffer)
Predicted band size: 58 kDaPrimary incubation was 1 hour. Detected by chemiluminescence.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUF60/FIR antibody (ab22819)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human prostate staining PUF60/FIR with ab22819 at 5 μg/ml
-
Immunocytochemistry/ Immunofluorescence - Anti-PUF60/FIR antibody (ab22819)ICC/IF image of ab22819 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22819, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUF60/FIR antibody (ab22819)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human skin staining PUF60/FIR with ab22819 at 5 μg/ml
-
Western blot - Anti-PUF60/FIR antibody (ab22819)Anti-PUF60/FIR antibody (ab22819) at 1 µg/ml + A431 lysate (35µg protein in RIPA buffer).
Predicted band size: 58 kDa
Observed band size: 58 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
