Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PCGF6 antibody [EPR19450] (ab200038)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19450] to PCGF6
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-PCGF6 antibody [EPR19450]
    See all PCGF6 primary antibodies

  • Description

    Rabbit monoclonal [EPR19450] to PCGF6

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    IP
    Mouse
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse testis, thymus, ovary and embryo lysates; F9, C6, RAW 264.7 and PC-12 whole cell lysates; rat testis lysate. ICC/IF: F9 cells. IP: F9 whole cell lysate.

  • General notes

     

     

     This product was previously labelled as RING6a

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PCGF6 antibody [EPR19450] (ab200038)
    Western blot - Anti-PCGF6 antibody [EPR19450] (ab200038)
    All lanes : Anti-PCGF6 antibody [EPR19450] (ab200038) at 1/1000 dilution

    Lane 1 : Mouse testis lysate
    Lane 2 : Mouse thymus lysate
    Lane 3 : Mouse ovary lysate
    Lane 4 : Mouse embryo lysate
    Lane 5 : F9 (Mouse embryonic testicular cancer cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 40 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-4: 3 minutes; Lane 5: 10 seconds.

  • Western blot - Anti-PCGF6 antibody [EPR19450] (ab200038)
    Western blot - Anti-PCGF6 antibody [EPR19450] (ab200038)
    All lanes : Anti-PCGF6 antibody [EPR19450] (ab200038) at 1/1000 dilution

    Lane 1 : Rat testis lysate
    Lane 2 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 3 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 40 kDa
    Observed band size: 42 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1/2 : 3 minutes; Lane 3/4: 30 seconds.

  • Immunocytochemistry/ Immunofluorescence - Anti-PCGF6 antibody [EPR19450] (ab200038)
    Immunocytochemistry/ Immunofluorescence - Anti-PCGF6 antibody [EPR19450] (ab200038)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized F9 (Mouse embryonic testicular cancer cell line) cells labeling PCGF6 with ab200038 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on F9 cells. The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab200038 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.

    -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary  antibody at 1/1000 dilution.

  • Immunoprecipitation - Anti-PCGF6 antibody [EPR19450] (ab200038)
    Immunoprecipitation - Anti-PCGF6 antibody [EPR19450] (ab200038)

    Ab200038 Immunoprecipitating PCGF6 in F9(mouse embryonal carcinoma) whole cell lysate. 350µg of cell lysate was incubated with primary antibody (2 µg/ml). For western blotting Ab200038 (1/1000) was used to confirm successful immunoprecipation. For detection VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000.

    Lane 1: (Input) F9(mouse embryonal carcinoma) whole cell lysate 10μg
    Lane 2: (+) F9 whole cell lysate
    Lane 3: (-) Rabbit monoclonal IgG (ab172730) instead of ab200038 in F9 whole cell lysate

  • Anti-PCGF6 antibody [EPR19450] (ab200038)
    Anti-PCGF6 antibody [EPR19450] (ab200038)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.