Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸ 10 µl 120 614 ₸

Order now and get it on Tuesday March 09, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11034] to DEK
Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
Reacts with: Human

Product name

Anti-DEK antibody [EPR11034]
See all DEK primary antibodies
Description

Rabbit monoclonal [EPR11034] to DEK
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues in Human DEK (P35659).
Positive control
- WB: 293T, HeLa, Jurkat and K562 cell lysates. IHC-P: Human breast and gastric carcinoma tissues. ICC/IF: 293T cells. IP: HeLa cell lysate. Flow Cyt: HeLa cells.
General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11034
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DEK antibody [EPR11034] (ab166624)

Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling DEK with ab166624 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-DEK antibody [EPR11034] (ab166624)

All lanes : Anti-DEK antibody [EPR11034] (ab166624) at 1/1000 dilution

Lane 1 : 293T cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : Jurkat cell lysates
Lane 4 : K562 cell lysates

Lysates/proteins at 10 µg per lane.

Predicted band size: 43 kDa
Flow Cytometry - Anti-DEK antibody [EPR11034] (ab166624)

Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling DEK (red) with ab166624 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
Immunocytochemistry/ Immunofluorescence - Anti-DEK antibody [EPR11034] (ab166624)

Immunofluorescence analysis of 293T cells labeling DEK with ab166624 at 1/100 dilution.
Immunoprecipitation - Anti-DEK antibody [EPR11034] (ab166624)

Immunoprecipitation of DEK in pellet from HeLa cell lysate using ab166624 at 1/10 dilution, followed by WB detection of DEK using as secondary an HRP-conjugated anti-rabbit IgG, which preferentially detects the non-reduced form of rabbit IgG.

Anti-DEK antibody [EPR11034] (ab166624) at 1/1000 dilution + immunoprecipitation pellet from HeLa cell lysate at 10 µg
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DEK antibody [EPR11034] (ab166624)

Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling DEK with ab166624 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-DEK antibody [EPR11034] (ab166624)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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