Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DEK antibody [EPR11034] (ab166624)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11034] to DEK
  • Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-DEK antibody [EPR11034]
    See all DEK primary antibodies

  • Description

    Rabbit monoclonal [EPR11034] to DEK

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues in Human DEK (P35659).

  • Positive control

    • WB: 293T, HeLa, Jurkat and K562 cell lysates. IHC-P: Human breast and gastric carcinoma tissues. ICC/IF: 293T cells. IP: HeLa cell lysate. Flow Cyt: HeLa cells.

  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11034

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DEK antibody [EPR11034] (ab166624)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DEK antibody [EPR11034] (ab166624)

    Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling DEK with ab166624 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-DEK antibody [EPR11034] (ab166624)
    Western blot - Anti-DEK antibody [EPR11034] (ab166624)
    All lanes : Anti-DEK antibody [EPR11034] (ab166624) at 1/1000 dilution

    Lane 1 : 293T cell lysates
    Lane 2 : HeLa cell lysates
    Lane 3 : Jurkat cell lysates
    Lane 4 : K562 cell lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 43 kDa

  • Flow Cytometry - Anti-DEK antibody [EPR11034] (ab166624)
    Flow Cytometry - Anti-DEK antibody [EPR11034] (ab166624)

    Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling DEK (red) with ab166624 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

  • Immunocytochemistry/ Immunofluorescence - Anti-DEK antibody [EPR11034] (ab166624)
    Immunocytochemistry/ Immunofluorescence - Anti-DEK antibody [EPR11034] (ab166624)
    Immunofluorescence analysis of 293T cells labeling DEK with ab166624 at 1/100 dilution.
  • Immunoprecipitation - Anti-DEK antibody [EPR11034] (ab166624)
    Immunoprecipitation - Anti-DEK antibody [EPR11034] (ab166624)
    Immunoprecipitation of DEK in pellet from HeLa cell lysate using ab166624 at 1/10 dilution, followed by WB detection of DEK using as secondary an HRP-conjugated anti-rabbit IgG, which preferentially detects the non-reduced form of rabbit IgG.

    Anti-DEK antibody [EPR11034] (ab166624) at 1/1000 dilution + immunoprecipitation pellet from HeLa cell lysate at 10 µg
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DEK antibody [EPR11034] (ab166624)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DEK antibody [EPR11034] (ab166624)

    Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling DEK with ab166624 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-DEK antibody [EPR11034] (ab166624)
    Anti-DEK antibody [EPR11034] (ab166624)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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